| Enzyme Function: | rhamnogalacturonase |
| Accession number: | AN9134.2 |
| GenBank ID: | DQ490522 |
| FGSC number: | 10130 |
| Average molecular weight (clone, calculated): | 56.748 kDa |
| AA length (clone): | 535 aa |
| DNA length (clone): | 1608 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGTACGTCAGTCGCCTTTTGCTCTTTCTGGC CCCTTTGCTGGTCAAGGGTCAGCTCTCTGGCAGCGTCGGTCCTCTTACAT CGGTCAGCTCCAAGAGCCAAACCAAGACCTGCAATGTCCTCGATTATGGC GCCGTAGCGGACAAGTCTACGGATATTGGCCCTGCCTTGTCATCCGCCTG GGATGAATGTGCCGACGGTGGGGTTGTCTACATCCCTCCTGGCGACTACG CGATTGAGACATGGGTCAAACTGTCTGGTGGAAAGGCCTGTGCCATCCAG CTCGACGGTATCATCTACCGCACCGGCACCGACGGCGGGAACATGATTAT GATCGAACACACCTCGGACTTCGAGTTCTTCAGCAGCACCTCCAAGGGCG CATTCCAGGGCTACGGATACGAGTTCCATGCCAAGGGCTCGTCGGACGGA CCACGGATTCTCAGACTCTACGACGTGTCGGACTTTTCCGTGCATGATGT CGCCCTCGTTGATTCGCCGCTCTTCCACTTCTCCATGGACACCTGCTCCA ACGGAGAGGTATACAACATGGCCATCCGCGGTGGCAATATGGGCGGCCTG GATGGAATTGACGTTTGGAGCACAAACGTCTGGATCCACGACGTCGAGGT GACAAACAAAGACGAATGCGTCACCGTTAAGAGCCCCTCCAAGAACATCC TCGTCGAGAACATATACTGCAACTGGAGTGGAGGCTGTGCCATGGGCTCC CTAGGCACCGACACTGATATTTCTGACATTGTGTACCGGAACGTCTACAC CTGGAAATCGAACCAGATGTATATGGTCAAGAGCAACGGGGGCAGCGGGA CCGTGTCCAACCTTGTGCTGGAGAACTTCATCGGTCACGGAAACGCCTAC TCGCTCGACATCGATAGCGCCTGGAGCAGCATGAGCACGATTGAAGGCGA CGGTGTTGAGTTGAAGAACGTGACAATTCGCAACTGGAAGGGAACCGAAG CAGACGGATCCCAGCGAGGCCCGATCAAGGTGAAGTGTGCATCCGGAGCA CCTTGCACTGACGTTACGGTTGAGGACTTTGCAATGTGGACCGAGTCAGG AGATGAACAGACGTATGTCTGTGAGAACGCGTTCGGCGACGGATTCTGTC TCGCAGACGGTGACGGCACCTCGACCTTCACCACGACTCTGACCGCCTCG GCGGCGCCCTCTGGTTACTCCGCACCCAGCATGGACGCCGACCTCGAGAC CGCCTTTGGTACTGACAGCGAGATCCCCATCCCCACCATTCCAACCTCCT TCTACCCAGGTGCCACCCCCTACAGCGCGCTTGCCGGAGCTTCAGTCTCT TCAAGTCAAGTACCCGCTGCATCTTCCAGTGCTGAGGCCAAATTCGTCGC CAGTCCCGCGACTAGCTCTCCCACCGCGACTTCCACGGCCATCTCTTCCG TCGATCCCGTTTCGGCGGCCACAACGACTGCGACTTCCCACGGGCATGGG AAATCTCACCACAAACATCAGTGTCGTGCCCACCGCCATCTAGAACAAAA ACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATC ATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMYVSRLLLFLAPLLVKGQLSGSVGPLTSVSSKSQTKTCNVLDYG AVADKSTDIGPALSSAWDECADGGVVYIPPGDYAIETWVKLSGGKACAIQ LDGIIYRTGTDGGNMIMIEHTSDFEFFSSTSKGAFQGYGYEFHAKGSSDG PRILRLYDVSDFSVHDVALVDSPLFHFSMDTCSNGEVYNMAIRGGNMGGL DGIDVWSTNVWIHDVEVTNKDECVTVKSPSKNILVENIYCNWSGGCAMGS LGTDTDISDIVYRNVYTWKSNQMYMVKSNGGSGTVSNLVLENFIGHGNAY SLDIDSAWSSMSTIEGDGVELKNVTIRNWKGTEADGSQRGPIKVKCASGA PCTDVTVEDFAMWTESGDEQTYVCENAFGDGFCLADGDGTSTFTTTLTAS AAPSGYSAPSMDADLETAFGTDSEIPIPTIPTSFYPGATPYSALAGASVS SSQVPAASSSAEAKFVASPATSSPTATSTAISSVDPVSAATTTATSHGHG KSHHKHQCRAHRHLEQKLISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGTACGTCAGTCGCCTTT (Pml I) TACAGTCTAGATGGCGGTGGGCACGAC (Xba I) |
| pH optimum: | n.d. |
| Specific activity (Substrate): | n.d. |
| T optimum: | n.d. |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on linseed RG produces (RG)2 and (RG)3 oligomers 2 silent mutations in Pichia clone: nucleotide 147 is T instead of C and nucleotide 1266 is C instead of T |
| References: | not yet available |
rhamnogalacturonase