Enzyme Function:alpha-galactosidase
Accession number: AN9035.2
GenBank ID: DQ490520
FGSC number: 10128
Average molecular weight
(clone, calculated): 		83.966 kDa
AA length (clone): 754 aa
DNA length (clone): 2265 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGACCAATTCCAAAGCCAGATATGTCTTAGA GCCAGTGCAGGTCACTGGCACAACTTTTGCCCTCAACGGTACCACACTCT CCTACCAGTTCCACGTCGATGAGCCCTCCGCCGACTTGCGGTCCGACCAT TTCGGCGGCTCAATCAGCGGCCCTATCCCCGTCGATCCTGAGCCCATCGT TGATGGGTGGACAGGGATGCCAGACCGCGTACGGCGCGAGTTCCCTGATC AAGGCAGAGGAGACTTTCGGGTACCTGCGTTTAGGATCCGGCAGGCTGAA GGGCATACAGTCTCGGCGTTTCGGTATCGGGAGCATGAAATCGTTCCAGG AAAGGAAGTCTCTGCGTCTGGACTACCAGGGGTCTTTGGGGATGCTCATG ATGCGACCACGCTAATTGTACGGTTGGTTGATCCATATAGCGACTTGGCC GCGGAGTTGAAGTATACGGTCTTTCCGAAGTATGATACCGTGGTACGCAG TGCAAGTATCACGAATAAGAGCAATAGTGACGTAACAATCGAGTCGCTTG CAAGTCTCAGTGTTGATTTTCCTTTTGACGAACTCGAGATGATCGGACTA CGAGGCGACTGGGCGAGAGAGGCTCATCGTCTGCGGCGCAAGGTGGACTA CGGGGTACAGAGTTTTGGCAGCACAACCGGATTCTCCTCGCATCTGCATA ACCCGTTCGTCGCTCTTGTGCACCCGTCCACCACCGAATCACAAGGTGAA GCATGGGGGTTCTCGCTCATCTACAGCGGATCTTTCACTATCAATGTCGA GAAATCCTCGCAAGGGCTGACGCGCGTCTCCATCGGACCGAGCCAACTGT CTTGGACTCTCAAACCTGGTGAGACATTTGACTCGCCCGAGTGTGTTGCT GTCTACTCCAGCACTGGGATTGGAGGGATGTCCCGTTCTCTCCATGGGCT GTATCGGAAACACCTAATGAAGAGCAAGTTCGCGACCGAAGACCGCCCCG TGCTCTTGAATAGCTGGGAGGGGCTGTATTTTCATATCGACCAGGACCGG ATGTACCGTCTCGCACAGGAGGCGGCGGCGTTGGGCGTCAAGCTGCTCGT CATGGATGACGGCTGGTTTGGGGACGAGCATCCGCGGACCTCTGATGATG CCGGCTTGGGCGATTGGATCCCCAATCCGGCCAGATTCCCTGACGGGCTG ACGCCGTTAGTGAACAGGATCACTGCGTTAAAGGTCGCGAATTCGCAGAG AAACATGCGCTTCGGGATCTGGGTTGAGCCAGAGATGGTCAACCCTCGAT CCACGCTCTACCAGCAACACCCCGACTGGGTTCTTCATGCTGGCAATTAT CCACGCACTGAGCAGCGCAATCAGCTTGTGCTCAATATGAGTCTGCCGGA GGTGCAGGAGTTCATTATCAGCGCGATGTCGGAGATCCTAAACGCCGCTG ACATTACATATGTCAAGTGGGACCACAACCGCGGCCTCGCAGAGACACCA TCTCCATGCGCAAACCACGCCTATATGCTAGGCGCCTACCGCGTGTTTGA TGTCCTCACCACCCGCTTTCCTAATGTCATCTGGGAGGGCTGCGCATCGG GCGGGGGCCGCTTCGACCCAGGAATCCTGCAGTACTTCCCACAAGTATGG ACATCCGACGACAGCGACGCGGTCGAGCGGATCTTCATCCAGTTCGGTAC CTCTCTTGCATACCCGGCCAGCGCAATGGGGGGGCATATATCGTCCGTGC CAAATCACCAGACCGGCCGCACCACACCGCTGACCTTCCGCGCCCACGTC GCCATGATGTGCGGCTCCTTCGGCCTAGAACTCGACCCAGCCCACTTGAC CGATTCCGAAAGAAGAGACATTCCCGGCTTGATTGCTTTAGCGGAAAAGA TCAGCCCGATTGTGGTTAAGGGCGACTTGTGGCGGTTAGCTTTACCGGAG GATTCGAACTGGCCAGCGGCGCTGTTCCTTTCCGAGAACCGGACACAGGG CGTGTTGTTCTTCTTCCAATTGGCGCCAATGGTCAACCATTCCTTGCCAC GGGTGAGATTGCAGGGTTTAGAGGACGGGGCGCTGTATCGGGTTGATGGA GAGGGGCCGTATTCCGGGTCGATGCTGATGAATCTGGGGTTGCAGTATTC GTTCAGGGGTGATTATGGTAGTAGACTTGCCTTCATAGAGAGAGATCTAG AACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCAT CATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMTNSKARYVLEPVQVTGTTFALNGTTLSYQFHVDEPSADLRSDH FGGSISGPIPVDPEPIVDGWTGMPDRVRREFPDQGRGDFRVPAFRIRQAE GHTVSAFRYREHEIVPGKEVSASGLPGVFGDAHDATTLIVRLVDPYSDLA AELKYTVFPKYDTVVRSASITNKSNSDVTIESLASLSVDFPFDELEMIGL RGDWAREAHRLRRKVDYGVQSFGSTTGFSSHLHNPFVALVHPSTTESQGE AWGFSLIYSGSFTINVEKSSQGLTRVSIGPSQLSWTLKPGETFDSPECVA VYSSTGIGGMSRSLHGLYRKHLMKSKFATEDRPVLLNSWEGLYFHIDQDR MYRLAQEAAALGVKLLVMDDGWFGDEHPRTSDDAGLGDWIPNPARFPDGL TPLVNRITALKVANSQRNMRFGIWVEPEMVNPRSTLYQQHPDWVLHAGNY PRTEQRNQLVLNMSLPEVQEFIISAMSEILNAADITYVKWDHNRGLAETP SPCANHAYMLGAYRVFDVLTTRFPNVIWEGCASGGGRFDPGILQYFPQVW TSDDSDAVERIFIQFGTSLAYPASAMGGHISSVPNHQTGRTTPLTFRAHV AMMCGSFGLELDPAHLTDSERRDIPGLIALAEKISPIVVKGDLWRLALPE DSNWPAALFLSENRTQGVLFFFQLAPMVNHSLPRVRLQGLEDGALYRVDG EGPYSGSMLMNLGLQYSFRGDYGSRLAFIERDLEQKLISEEDLNSAVDHH HHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGACCAATTCCAAAGCCA (Pml I) TACAGTCTAGATCTCTCTCTATGAAGGCAAGTCT (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: not active on PNP-alpha-galactoside, raffinose
References: not yet available