| Enzyme Function: | exo-polygalacturonase |
| Accession number: | AN8761.2 |
| GenBank ID: | DQ490519 |
| FGSC number: | 10127 |
| Average molecular weight (clone, calculated): | 51.283 kDa |
| AA length (clone): | 467 aa |
| DNA length (clone): | 1404 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGAAGCTCTCACACCTCCTCACCTCCGCCGT GAGCGTCCTCTCCCTCGGCCTCACAGTCGAAGGCCACTTCTCACGCTCTC GAAATGATGCCGTAGGCCCCAAGCGGCCTTTCAAGCCTCTCCCCTACAGC CACCCACGCAAGAAAGTCTGCCATGTGCGCTCCCATGGCGACGGCCGCGA CGACTCGGCGTTCATCCTCTCCGCCTTGAAGAGCTGCAATAACGGCGGAA AGGTCGTCTTCGCCGAAGAGAAGGAGTATACCATCGGCACGGCTCTAGAT CTGACGTTCCTGAAGCATGTTGATCTTGAAATCCTCGGCCGCATTCAGTT CACAAACGACACGGATTACTGGCAAGCCAACTCTTTCAAACACACGTTCC AGAACGCAACGACCTTCTTCCAGCTTGGCGGCGAAGACGTGAACGTCTAT GGCGGGGGCACTCTCGACGGCAATGGCCAAATCTGGTACGACCTGTACGC TGAGGACCCGCTTATCCTGCGCCCTATTTTGTTTGGAGTGATCGGCCTGC ACGGCGGGACAATTGGGCCGCTGAAGTTGCGCTACTCGCCGCAGTGGTAC CAGCTTGTTGCGAACAGCTCGGATGTGCTCTTCGACGGCATCGACATTTC CGGGTACAGCAAAAGCGAGAACGAGGCGAAGAACACGGATGGGTGGGATA CGTACCGGTCTAAGAATATTGTTATTCAGAATTCGGTTATCAACAATGGG GATGACTGCGTCTCCTTCAAACCGAACAGTACCGAAATACTTGTCCAGAA CCTGTACTGCAATGGCTCGCACGGTATCTCCGTTGGTTCGCTAGGTCAAT ACATTGGCGAGGTTGATATCGTCAAGAACGTGCTCGTCTATAACATCTCT ATGTATAATGCTTCTGATATGGCTCGCATCAAGGTCTGGCCGGGCGTCGC ATCCGCCATGTCCGAGGACCTTCAGGGCGGAGGCGGTCTCGGATCAGTCT CAAATATCACCTACGAGGACATGTACATTGAGAACGTCGACTGGGCGATT GAAATCACACAGTGCTATGGCCAAAAGAACATGACCTTGTGCAACGAGTA CCCGAGTAACCTGACCATCTCTGACGTCTACATTTCCAATATGTACGGAA CGACATCGTCAGCGCGGGATCCGAACATCGGCACAATTGTTTGCTCGAGT CCGGATGTTTGCTCCAATATCTATGTTGAGAATATTGATGTTGTCAGCCC GAGCGGTACAAATGACTTTATTTGCACGAATGTCAACGAGAGTCTCCTGC AGGTTAACTGTACCTCTGGCGCGGCCGCCAGCTTTCTAGAACAAAAACTC ATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCA TTGA |
| Amino Acid Sequence (clone): | SMNSRDMKLSHLLTSAVSVLSLGLTVEGHFSRSRNDAVGPKRPFKPLPYS HPRKKVCHVRSHGDGRDDSAFILSALKSCNNGGKVVFAEEKEYTIGTALD LTFLKHVDLEILGRIQFTNDTDYWQANSFKHTFQNATTFFQLGGEDVNVY GGGTLDGNGQIWYDLYAEDPLILRPILFGVIGLHGGTIGPLKLRYSPQWY QLVANSSDVLFDGIDISGYSKSENEAKNTDGWDTYRSKNIVIQNSVINNG DDCVSFKPNSTEILVQNLYCNGSHGISVGSLGQYIGEVDIVKNVLVYNIS MYNASDMARIKVWPGVASAMSEDLQGGGGLGSVSNITYEDMYIENVDWAI EITQCYGQKNMTLCNEYPSNLTISDVYISNMYGTTSSARDPNIGTIVCSS PDVCSNIYVENIDVVSPSGTNDFICTNVNESLLQVNCTSGAAASFLEQKL ISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGAAGCTCTCACACCTCCTC (Pml I) TACAGGCGGCCGCGCCAGAGGTACAGTTAACCTG (Not I) |
| pH optimum: | 4.4 |
| Specific activity (Substrate): | pectic acid |
| T optimum: | 48 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on pectic acid, less active on citrus pectin, removes individual GalA residues. Degrades end-labeled GalA oligomers to labeled GalA2 with slow conversion to labeled GalA. |
| References: | not yet available |
exo-polygalacturonase