| Enzyme Function: | pectate lyase |
| Accession number: | AN8453.2 |
| GenBank ID: | DQ490518 |
| FGSC number: | 10126 |
| Average molecular weight (clone, calculated): | 30.810 kDa |
| AA length (clone): | 288 aa |
| DNA length (clone): | 867 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGTTCATCAAGAACGGCCTGCTTCTCTCCCT GGCGACCAGCGTGCTGGCCACGCACGGCGCCCGCTCCCACTCCCAGGCCG CCACCAGCAAGCTCTCGAAGCGCTTCACCTTCCCTATCCCCAACTCTGAG GGCAGCGTCACCCTCGACGAGGTCTATGAGATCGACGGCGAGACCTTTGA CGGCGGCATGAAAACCTACGGCCGTGGCGTCTCCTGCACTGGTCAAGACG AAGGCGGCGACTCTGACGCCGTCTTCATCGTCAAGAACGGCGGTACCCTC AAGAACGTCATCATCGGCTCGGACCAGATCGAGGGTGTCCACTGCGAGGG CTCCTGCACTATCGAGAACGTCTGGTGGGAGGCCGTTTGCGAGGACGCCC TCTCCCTCAAGACTGGTGACGGCCCCTTCAACGTCGTTGGTGGTGGTGCT CAGGGCGCCGACGACAAGGTCATCCAGCACAACGGTGGCGGTACCGTCAG CATTTCCGACTTCACCGTCTACGACTTCGGCAAGCTCTACCGCTCCTGCG GAAACTGTGGCGACCAGTACGAGCGTCACGTTGTCATTGAGGGAGTCACC GCCGTTGACGGCAAGTACCTTGTTGGCATCAACAGCAACTACGGCGACAC TGCCACTATTGACAGCGCTACTTGCGCCACGGATGTCAAGACCATCTGCG CTGAGTACAAGGGCACTGACAACAACGACGAGGAGCCCGAGGAGGTCAGC GATGGCCCTAGCGACTACTGCATCTACACTGAGCCGCTCTCTGAGTGTCT AGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATC ATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMFIKNGLLLSLATSVLATHGARSHSQAATSKLSKRFTFPIPNSE GSVTLDEVYEIDGETFDGGMKTYGRGVSCTGQDEGGDSDAVFIVKNGGTL KNVIIGSDQIEGVHCEGSCTIENVWWEAVCEDALSLKTGDGPFNVVGGGA QGADDKVIQHNGGGTVSISDFTVYDFGKLYRSCGNCGDQYERHVVIEGVT AVDGKYLVGINSNYGDTATIDSATCATDVKTICAEYKGTDNNDEEPEEVS DGPSDYCIYTEPLSECLEQKLISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGTTCATCAAGAACGGCCT (Pml I) TACAGTCTAGACACTCAGAGAGCGGCTCAGT (Xba I) |
| pH optimum: | 7.8 |
| Specific activity (Substrate): | pectic acid |
| T optimum: | 22 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on pectic acid, Ca2+ is cofactor, produces GalA2 and GalA3 with unsaturated GalA at the NR end |
| References: | not yet available |
pectate lyase