Enzyme Function:beta-xylosidase/alpha-arabinosidase
Accession number: AN8401.2
GenBank ID: XM_676578
FGSC number: 10125
Average molecular weight
(clone, calculated): 		85.499 kDa
AA length (clone): 791 aa
DNA length (clone): 2376 bp
DNA source: genomic DNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGGCCGTATTCAAATCCTGGAACCTTGCCTT ACTCTCCTCTCTCTTCATCCCCGCCCTGTGTCAGTCCAACTACCCGGACT GCACAACGGGCCCTCTCTCTGAACTTCCCATATGCGATACCAGCCTGTCC CCTCTCGAACGAGCAAAATCCCTTGTCTCTGCCCTGACACTCGAAGAGAA AATCAACAACACGGGCCACGAAGCAGCTGGAAGCTCCCGCTTAGGTCTCC CTGCGTACAACTGGTGGAACGAAGCCCTGCACGGAGTCGCTGAAAAACAC GGCGTGAGTTTCGAAGAGTCTGGTGATTTTAGCTACGCGACATCCTTCCC AGCACCCATTGTGCTCGGTGCCGCGTTCAACGATGCGCTCATCAGGAGAG TCGCTGAGATAATCTCTACTGAGGCGCGGGCGTTTTCGAACTCCGACCAC GCAGGGATCGATTACTGGACGCCGAATGTAAATCCATTTAAAGATCCAAG ATGGGGTCGCGGGCAGGAAACACCCGGTGAAGACCCGCTGCACTGCTCGC GCTATGTCAAAGAGTTTGTGGGCGGCTTGCAGGGCGACGATCCCGAGAAG CCGAAGGTCGTTGCTACGTGCAAGCATCTCGCGGCATATGATCTGGAAGA ATGGGGCGGAGTCAGCCGGTTCGAGTTCGACGCAAAGGTGTCCGCTGTCG ATCTTCTCGAGTACTATCTTCCGCCTTTCAAGACCTGCGCCGTTGACGCA AGCGTCGGCGCGTTCATGTGCAGTTATAATGCCCTCAATGGGGTTCCCGC ATGTGCGGATCGATATCTCCTCCAGACAGTGCTGCGTGAGCACTGGGGAT GGGAGGGTCCAGGACACTGGGTGACCGGTGATTGTGGTGCTGTTGAGCGC ATCCAGACGTACCACCACTACGTTGAGAGTGGACCTGAAGCCGCGGCTGC GGCGCTGAATGCCGGCGTGGACCTGGACTGTGGGACCTGGCTTCCAAGCT ACCTGGGGGAGGCGGAAAGGCAGGGCTTGATCAGCAATGAGACGCTGGAC GCGGCGTTGACGAGACTATATACGAGTCTCGTACAGCTGGGGTACTTTGA TCCGGCCGAGGGTCAACCGCTGCGGTCCCTGGGCTGGGACGATGTAGCGA CGAGCGAGGCGGAGGAGCTCGCAAAGACTGTCGCGATCCAGGGCACCGTG CTGCTGAAGAATATCGACTGGACCCTTCCACTGAAGGCGAACGGCACACT GGCACTTATTGGGCCCTTTATCAATTTCACCACCGAGCTGCAGAGCAATT ATGCGGGCCCGGCCAAGCACATCCCGACCATGATCGAGGCAGCAGAGAGA CTCGGCTACAACGTTCTCACCGCGCCAGGGACAGAGGTGAATTCGACAAG CACAGACGGATTCGATGATGCCCTCGCAATAGCTGCAGAGGCCGACGCCC TGATCTTCTTTGGCGGGATCGACAACACCGTCGAGGAGGAGTCGCTTGAT CGAACACGCATCGACTGGCCCGGGAACCAGGAGGAACTCATTCTGGAACT CGCCGAGCTGGGAAGACCGCTGACGGTCGTGCAGTTCGGCGGCGGCCAAG TTGACGACTCAGCGCTGCTGGCATCTGCCGGCGTCGGTGCGATTGTCTGG GCCGGGTATCCCAGCCAGGCCGGTGGCGCAGGGGTCTTTGATGTCCTGAC CGGTAAAGCAGCGCCCGCCGGTCGACTACCGATCACGCAGTATCCCAAGA GCTATGTCGACGAGGTTCCGATGACAGATATGAACCTGCAACCTGGAACA GATAATCCCGGCCGCACATATAGATGGTACGAAGACGCGGTCCTGCCATT CGGGTTCGGACTGCATTATACGACATTCAATGTCTCGTGGGCGAAGAAGG CATTTGGGCCGTACGATGCTGCCACCCTTGCCAGGGGCAAAAATCCCAGC TCGAACATTGTCGATACATTCTCCCTCGCAGTCACCAATACGGGAGACGT CGCTTCTGACTATGTTGCGCTGGTGTTCGCCAGCGCTCCTGAGCTCGGGG CTCAGCCTGCGCCGATCAAGACGCTGGTCGGGTACAGTAGGGCCAGTTTA ATCAAGCCTGGGGAGACAAGAAAAGTAGACGTCGAGGTTACCGTTGCGCC GCTGACGAGGGCGACTGAGGATGGCAGGGTGGTACTGTACCCAGGAGAGT ATACACTGCTTGTGGACGTCAACGATGAGTACCCGACAGCTAAGTTTGAG ATCAAGGGGGATGTGCAGGTGCTTGAAAAGTTTCCGTTGTCAGGTAACGA CAGTGATCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCG TCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMAVFKSWNLALLSSLFIPALCQSNYPDCTTGPLSELPICDTSLS PLERAKSLVSALTLEEKINNTGHEAAGSSRLGLPAYNWWNEALHGVAEKH GVSFEESGDFSYATSFPAPIVLGAAFNDALIRRVAEIISTEARAFSNSDH AGIDYWTPNVNPFKDPRWGRGQETPGEDPLHCSRYVKEFVGGLQGDDPEK PKVVATCKHLAAYDLEEWGGVSRFEFDAKVSAVDLLEYYLPPFKTCAVDA SVGAFMCSYNALNGVPACADRYLLQTVLREHWGWEGPGHWVTGDCGAVER IQTYHHYVESGPEAAAAALNAGVDLDCGTWLPSYLGEAERQGLISNETLD AALTRLYTSLVQLGYFDPAEGQPLRSLGWDDVATSEAEELAKTVAIQGTV LLKNIDWTLPLKANGTLALIGPFINFTTELQSNYAGPAKHIPTMIEAAER LGYNVLTAPGTEVNSTSTDGFDDALAIAAEADALIFFGGIDNTVEEESLD RTRIDWPGNQEELILELAELGRPLTVVQFGGGQVDDSALLASAGVGAIVW AGYPSQAGGAGVFDVLTGKAAPAGRLPITQYPKSYVDEVPMTDMNLQPGT DNPGRTYRWYEDAVLPFGFGLHYTTFNVSWAKKAFGPYDAATLARGKNPS SNIVDTFSLAVTNTGDVASDYVALVFASAPELGAQPAPIKTLVGYSRASL IKPGETRKVDVEVTVAPLTRATEDGRVVLYPGEYTLLVDVNDEYPTAKFE IKGDVQVLEKFPLSGNDSDLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGGCCGTATTCAAATC (Pml I) TACAGTCTAGATCACTGTCGTTACCTGACAAC (Xba I)
pH optimum: 4.4
Specific activity (Substrate): RAX
T optimum: 48
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on RAX and Xyl6 , but not on PNP-beta- xyloside
References: not yet available