Enzyme Function:alpha-fucosidase
Accession number: AN8149.2
GenBank ID: DQ490516
FGSC number: 10123
Average molecular weight
(clone, calculated): 		91.817 kDa
AA length (clone): 837 aa
DNA length (clone): 2514 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGCGGAAGACCACTCTGTTTCTGGCGGTCAC TTTTGCGACCTCGAATGCCCAAGGCAGAGCCCTAAGATCGTCCTCTCCCG CTACATATGGCACCACAGACGGAAGCGACTATATCCTGAAGACTGGCTAT CTGATCGGGAACGGGAAGCTTGGAGTAATCCCGTTCGGCCCACCAGACAC CGAGAAGCTGAACCTGAACGTCGACAGTCTGTGGTCCGGGGGTCCATTTG AGGTCGAGAACTATACTGGCGGCAACCCTTCGTCACCGATCTATGATGCG CTACCAGGGATCCGGGAGAGAATCTTTGAGAATGGAACCGGTGGCATGGA AGAATTACTCGGCAGTGGGAACCACTATGGTTCCAGCCGTGTGTTGGGGA ATATAACCATCGCCTTGGACGGAGTCGAAGCCTACAGCAAATACAAGAGA ACACTCGACTTATCTGATGGCGTCCACAGAACGAGTTTTACCATTGCCAA TCGCACAACGGCAGCGCTCAAGTCGTCCATTTTTTGCTCATATCCCGATC AAGTCTGCGTCTACCACCTTGAATCAGCATCAGATGCGCGTCTGCCCAAG GTGACAATCAGCATTGAGAATCTCCTTGTGAACCAGAGCCTCCTGCAAAC GTCTTGTGAGAGCGAGGCCAAACGGGCTGTTTTGCGGCATTCTGGCGTCA CGCAAGCCGGTCCACCAGAAGGGATGAAGTATGCTGCAGTTGCAGAGGTG GTAAATCCTCGATCTTCTGTGACAACATGTCTGGGCGAGGGTGCGCTTCA GATCTCCTCTCGCAAGAAGCAACTCACGATCATCATCGGCGCGGCGACGA ATTACGACCAGAAAGCAGGAAACGCGAAGAGTGGCTGGTCCTTCAAGAAT GCAAAAGACCCAGCATCAATCGTGGATGGAATTGCCTCTGCTGCTGGTTG GAAGGGTTATCAGAGGCTTCTTGACCGTCACGTCAAAGACTACAAGAAGC TAATGGGCGATTTCTCGCTCGAATTGCCGGATACGACAGACTCCGCGAGC AAAGACACATCTGAGCTGATTGAGAAGTACTCGTACGCCTCTGCTACCGG CAACCCGTATCTGGAGAACCTCCTCTTGGATTACGCCAGACACCTCCTGG TCAGTTCTTCCCGGCCCAACTCCCTACCCGCAAACCTCCAAGGCCGCTGG ACCGAATCGCTCACCCCGTCATGGAGCGCCGACTACCACGCGAACATCAA CTTGCAGATGAATTACTGGCTAGCAGACCAGACCGGGCTCGGGGAGACAC AGCACGCTCTATGGAATTATATGGCGGATACTTGGGTTCCCAGGGGCACA GAGACGGCACGCCTGCTATACAACGCAAGCGGATGGGTCGTGCATAACGA GATAAATATCTTTGGGTTTACGGCCATGAAGGAGGACGCGGGGTGGGCGA ACTACCCTGCAGCCGCCGCCTGGATGATGCAGCACGTCTGGGATAACTTC GACTACACCCATGACACAGCCTGGCTGGTATCGCAGGGTTACGCGCTCCT CAAAGGCATCGCATCATTCTGGCTCTCATCTTTGCAGGAGGACAAGTTCT TCAACGACGGCTCGCTCGTTGTGAACCCCTGTAACAGCCCCGAGACTGGG CCAACAACATTCGGCTGCACGCACTACCAGCAGCTAATACACCAGGTTTT TGAAACCGTGCTTGCAGCGCAGGAGTATATCCACGAGTCCGATACCAAGT TTGTCGACTCCGTGGCCTCGGCCCTGGAAAGACTAGACACAGGCCTGCAC CTATCTTCCTGGGGCGGTCTCAAGGAGTGGAAGCTGCCGGATAGTTACGG TTACGACAATATGAGTACGCATCGACATCTGTCACACTTAGCGGGATGGT ATCCGGGGTACTCGATTTCGTCCTTTGCGCACGGATATAGGAACAAGACG ATACAGGATGCGGTCAAAGAGACCCTAACAGCACGCGGGATGGGCAATGC GGCGGACGCCAATGCTGGATGGGCCAAAGTGTGGCGGGCTGCGTGTTGGG CACGCCTGAACGACTCTTCCATGGCGTACGATGAGCTGCGGTATGCGATT GACGAAAACTTCGTCGGGAATGGGCTGAGCATGTATTGGGGAGCGAGCCC GCCGTTCCAGATTGATGCGAACTTTGGCTTTGCAGGGGCAGTGCTGAGCA TGTTGGTGGTTGATTTGCCAACTCCAAGGTCCGATCCTGGGCAGAGGACC GTTGTTCTGGGGCCCGCGATACCGTCTGCATGGGGGGGAGGCAGGGCTAA GGGGTTACGATTGCGAGGTGGTGCGAAGGTAGATTTTGGCTGGGATAAAA GGGGTGTTGTCAATTGGGTGAATATCGTGAAGAGAGGGAAGGGGACAAGC AGAGTCAAGCTGGTCAATAAGGAAGGTGACATTCTTGCTGAGATTCTAGA ACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATC ATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMRKTTLFLAVTFATSNAQGRALRSSSPATYGTTDGSDYILKTGY LIGNGKLGVIPFGPPDTEKLNLNVDSLWSGGPFEVENYTGGNPSSPIYDA LPGIRERIFENGTGGMEELLGSGNHYGSSRVLGNITIALDGVEAYSKYKR TLDLSDGVHRTSFTIANRTTAALKSSIFCSYPDQVCVYHLESASDARLPK VTISIENLLVNQSLLQTSCESEAKRAVLRHSGVTQAGPPEGMKYAAVAEV VNPRSSVTTCLGEGALQISSRKKQLTIIIGAATNYDQKAGNAKSGWSFKN AKDPASIVDGIASAAGWKGYQRLLDRHVKDYKKLMGDFSLELPDTTDSAS KDTSELIEKYSYASATGNPYLENLLLDYARHLLVSSSRPNSLPANLQGRW TESLTPSWSADYHANINLQMNYWLADQTGLGETQHALWNYMADTWVPRGT ETARLLYNASGWVVHNEINIFGFTAMKEDAGWANYPAAAAWMMQHVWDNF DYTHDTAWLVSQGYALLKGIASFWLSSLQEDKFFNDGSLVVNPCNSPETG PTTFGCTHYQQLIHQVFETVLAAQEYIHESDTKFVDSVASALERLDTGLH LSSWGGLKEWKLPDSYGYDNMSTHRHLSHLAGWYPGYSISSFAHGYRNKT IQDAVKETLTARGMGNAADANAGWAKVWRAACWARLNDSSMAYDELRYAI DENFVGNGLSMYWGASPPFQIDANFGFAGAVLSMLVVDLPTPRSDPGQRT VVLGPAIPSAWGGGRAKGLRLRGGAKVDFGWDKRGVVNWVNIVKRGKGTS RVKLVNKEGDILAEILEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGCGGAAGACCACTCTGT (Pml I) TACAGTCTAGAATCTCAGCAAGAATGTCACCTT (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: removes alpha-fucose from cotton XG oligomers, not active on PNP-fucoside
References: not yet available