| Enzyme Function: | alpha-galactosidase |
| Accession number: | AN8138.2 |
| GenBank ID: | DQ490515 |
| FGSC number: | 10122 |
| Average molecular weight (clone, calculated): | 85.509 kDa |
| AA length (clone): | 778 aa |
| DNA length (clone): | 2337 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGTTCCGCTCAACGGCTACGGTGGCCGCAGC CACCGCCATGGGCCTGTTGACGGCCACCGGCCATGGCTCATTGGCGATTG CGCAGGGTACAACTGGTTCCAATGCGGTCGTCGTCGACGGGACGAACTTC GCTCTCAACGGAGCCAGCATGTCCTACGTCTTCCACGCCAACAGCACCAC CGGCGATCTGGTCTCTGACCACTTCGGCGCGACCATCAGCGGTGCCATTC CGGCTCCAAAGGAGCCAGCCGTCAACGGCTGGGTCGGGATGCCCGGTCGA ATCCGGCGCGAATTCCCCGACCAAGGGCGCGGCGATTTCCGGATCCCCGC GGTGCGCATCCGTCAAACGGCTGGATATACTGTCAGCGACCTGCAGTATC AGGGCCATGAGGTGGTCGACGGTAAACCGGCGTTGCCTGGCCTGCCGGCG ACATTCGGTGAGGCGGGGGATGTGACGACGTTGGTTGTACATCTGTACGA CAATTACAGTGCCGTCGCTGCGGACCTCTCGTACAGTGTGTTTCCAGAAT TTGATGCTGTGGTTAGAAGCGTCAACGTTACGAATAAAGGAAAGGGCAAT ATTACTATTGAGAATTTGGCGAGTCTCTCTGTAGACTTCCCGCTCGAGGA CTTGGACCTCGTCAGTTTGAGGGGTGATTGGGCGAGAGAGGCGAACCGAG AGAGGCGGAGAGTAGAGTATGGCATTCAAGGATTCGGAAGCAGCACCGGC TACTCCTCCCACCTGCACAACCCCTTCTTCGCCCTCGTGCACCCGTCTAC GACCGAATCCCAGGGCGAAGCATGGGGCTTCAACCTCGTCTACACCGGCT CCTTCTCCGCTCAAGTCGAGAAGGGATCCCAGGGTTTGACCCGCGCGTTG ATCGGCTTCAACCCCGACCAACTCTCCTGGAACTTGGGACCCGGCGAGAC CCTGACGTCCCCTGAATGTGTGTCTGTGTACAGCAAAGACGGAATCGGCG GCATGTCGCGCAAATTCCACCGTCTCTACCGCAAGCACCTCATCAGGAGC AAGTTCGCGACATCGGATCGGCCGCCCCTCTTGAACAGCTGGGAAGGCGT TTACTTTGACTTCAACCAGAGCAGTATCGAGACTCTTGCCGAGCAATCCG CTGCTCTTGGCATCCGCCTCTTCGTCATGGATGACGGCTGGTTTGGGGAC AAATACCCCCGTACCTCCGACAATGCAGGACTCGGCGACTGGACGCCGAA TCCGGATCGGTTCCCTAATGGACTGGAGCCGGTCGTCGAGGAGATCACAA ATCTGACCGTCAACGACACCTCCGCTGAGAAACTGCGATTCGGTATCTGG GTCGAGCCGGAGATGGTCAACCCTAACTCGAGCCTCTACCGGGAGCACCC CGACTGGGCCCTGCACGCTGGCGCCTACGCACGCACGGAGAGACGCAACC AGCTCGTCTTGAACCTGGCGCTCCCCGAAGTCCAAGAATATATCATCGAT TTCATGACAGACTTGCTCAACAGCGCGGACATCTCCTACATCAAGTGGGA TAACAACCGCGGCATCCACGAGGCCCCGTCTCCTAGTACCGACCACGAGT ATATGCTGGGTGTATACCGAGTCTTTGACACCTTGACAGCGCGATTCCCC GATGTGCTATGGGAGGGATGTGCCTCTGGTGGGGGCCGCTTCGACGCGGG TGTTTTGCATTACTTCCCGCAAATCTGGACGTCGGATAACACCGACGGCG TGGACCGAGTCACTATCCAATTCGGCACGAGTCTGGCCTATCCACCTTCC GCCATGGGTGCACATCTCTCCGCCGTCCCGAATCACCAGACGGGCCGCAC GGTGCCACTGGAGTTCAGAGCGCACGTCGCAATGATGGGCGGGTCGTTCG GACTGGAGTTGGATCCCGCCACACTTCAGGATGATCCAGACGTCCCTGAA CTTATTCAAATGGCAGAGAAGGTGAATCCTCTGGTCCTGAACGGGGATCT CTACCGCCTGCGTTTGCCTGAGGAGAGCCAGTGGCCGGCGGCACTGTTTG TGGCTGAAGATGGAAGCCAGGCTGTACTCTTCTACTTCCAGCTGTCGCCA AACGTCAATCACGCAGCGCCCTGGGTCAGGCTCCAGGGCCTTGATCCGGA GGCGAGCTACACTGTCGATGGGGATAAGACATATACTGGTGCGACCCTTA TGAACCTGGGTCTGCAGTACACATTTGATACGGAGTACGGGAGCAAAGTG GTCTTCTTAGAAAGGCATCTAGAACAAAAACTCATCTCAGAAGAGGATCT GAATAGCGCCGTCGACCATCATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMFRSTATVAAATAMGLLTATGHGSLAIAQGTTGSNAVVVDGTNF ALNGASMSYVFHANSTTGDLVSDHFGATISGAIPAPKEPAVNGWVGMPGR IRREFPDQGRGDFRIPAVRIRQTAGYTVSDLQYQGHEVVDGKPALPGLPA TFGEAGDVTTLVVHLYDNYSAVAADLSYSVFPEFDAVVRSVNVTNKGKGN ITIENLASLSVDFPLEDLDLVSLRGDWAREANRERRRVEYGIQGFGSSTG YSSHLHNPFFALVHPSTTESQGEAWGFNLVYTGSFSAQVEKGSQGLTRAL IGFNPDQLSWNLGPGETLTSPECVSVYSKDGIGGMSRKFHRLYRKHLIRS KFATSDRPPLLNSWEGVYFDFNQSSIETLAEQSAALGIRLFVMDDGWFGD KYPRTSDNAGLGDWTPNPDRFPNGLEPVVEEITNLTVNDTSAEKLRFGIW VEPEMVNPNSSLYREHPDWALHAGAYARTERRNQLVLNLALPEVQEYIID FMTDLLNSADISYIKWDNNRGIHEAPSPSTDHEYMLGVYRVFDTLTARFP DVLWEGCASGGGRFDAGVLHYFPQIWTSDNTDGVDRVTIQFGTSLAYPPS AMGAHLSAVPNHQTGRTVPLEFRAHVAMMGGSFGLELDPATLQDDPDVPE LIQMAEKVNPLVLNGDLYRLRLPEESQWPAALFVAEDGSQAVLFYFQLSP NVNHAAPWVRLQGLDPEASYTVDGDKTYTGATLMNLGLQYTFDTEYGSKV VFLERHLEQKLISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGTTCCGCTCAACGGC (Pml I) TACAGTCTAGATGCCTTTCTAAGAAGACCACT (Xba I) |
| pH optimum: | 3.5 |
| Specific activity (Substrate): | PNP-alpha-galactoside |
| T optimum: | 52 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on PNP-alpha-galactoside, raffinose, LBG, and GG releasing Gal |
| References: | not yet available |
alpha-galactosidase