Enzyme Function:alpha-L-arabinofuranosidase
Accession number: AN7908.2
GenBank ID: XM_676085
FGSC number: 10119
Average molecular weight
(clone, calculated): 		38.801 kDa
AA length (clone): 353 aa
DNA length (clone): 1062 bp
DNA source: genomic DNA
DNA Sequence (clone): TCGATGAATTCACGTGCAATGAGAAACCTCAGTACCTGGCCCACATTCGC AGCCCTCCTTTGGTCTGCTCCAAGGGTCCTGGCGCAGTGCGGTCTCCCGT CAACGTACAGCTGGACGTCGACTGGCCCGCTCGCTGAACCCAAGGACGGA TGGGCATCACTCAAGGACTTCACTGCGGTCCCGTACAATGGGCAGTATCT TGTGTACGCAACTTATCATGATACGGGCACGAGCTGGGGCTCCATGAATT TCGGCCTCTTCTCGAACTGGTCCGACATGGCCACGGCGAGCCAGAACGCA ATGACTCAGTCGACTGTCGCGCCCACGCTATTCTACTTCGAGCCCAAGGA TGTCTGGATCCTCGCGTACCAATGGGGCCCTACTGCATTTTCCTACCTGA CGTCGAGTGATCCAACCGATGCCAATGGTTGGTCATCACCCCAACCGCTC TTTTCCGGGTCAATTTCAGACTCAGACACCGGGGTCATTGACCAGACAGT CATCGGCGACAGTACAACAATGTACCTCTTTTTCGCGGGAGACAACGGCC GCATCTATCGCGCCAGTATGCCCATCGACCAATTCCCCGGCGATTTCGGT ACCGAGTCCGAGATTATCCTGTCAGATGAAAGAAACAACTTGTTCGAAGC TGTCCAGGTGTACACGGTCTCAGGTCAAAGCAAAGACACATACCTAATGA TTGTCGAAGCGATCGGGGCACAGGGGCGCTATTTCCGGTCATTCACTGCC GACAGCCTTGGTGGCTCATGGACACCGCAGGCGGCCACTGAGAGCGCACC TTTTGCTGGCAAAGCCAACAGCGGTGCCACCTGGACGGACGATATCAGCC ATGGCGATCTTGTGCGCAGTACCCCGGATCAGACAATGAGCATCGACCCT TGCAACCTGCAGCTCCTCTATCAGGGCCGAGATCCCAGCCTCAACCCCGG CTACGACCTTCTGCCATATCGGCCAGGGCTCCTCACCCTGAATCTAGAAC AAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCAT CATCATCATTGA
Amino Acid Sequence (clone): SMNSRAMRNLSTWPTFAALLWSAPRVLAQCGLPSTYSWTSTGPLAEPKDG WASLKDFTAVPYNGQYLVYATYHDTGTSWGSMNFGLFSNWSDMATASQNA MTQSTVAPTLFYFEPKDVWILAYQWGPTAFSYLTSSDPTDANGWSSPQPL FSGSISDSDTGVIDQTVIGDSTTMYLFFAGDNGRIYRASMPIDQFPGDFG TESEIILSDERNNLFEAVQVYTVSGQSKDTYLMIVEAIGAQGRYFRSFTA DSLGGSWTPQAATESAPFAGKANSGATWTDDISHGDLVRSTPDQTMSIDP CNLQLLYQGRDPSLNPGYDLLPYRPGLLTLNLEQKLISEEDLNSAVDHHH HHH
Primers Used (Restriction sites): GAAAGCACGTGCAATGAGAAACCTCAGTACCTG (Pml I) TACAGTCTAGATTCAGGGTGAGGAGCCCT (Xba I)
pH optimum: 5.4
Specific activity (Substrate): PNP-alpha-arabinofuranoside
T optimum: 47
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: MM
Description: active on PNP-alpha-arabinofuranoside, sugar beet arabinan, RAX
References: not yet available