| Enzyme Function: | pectate lyase |
| Accession number: | AN7646.2 |
| GenBank ID: | DQ490513 |
| FGSC number: | 10118 |
| Average molecular weight (clone, calculated): | 38.426 kDa |
| AA length (clone): | 355 aa |
| DNA length (clone): | 1068 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGCAGAACCTCAAGTTCCTCATCGCCGCCGT CAGCTGCCTCGGGCCGGCCCTTGCAGCTCCGACTCCTACCAGCAACTTTA TCAACAGCAACTTCAACAAGCGTGCTTCCGTTGAAGATTCCGCCTTTGGC TATGCCAGCCTGAACGGCGGTACTACCGGCGGTGCCGGCGGTACCACAAC CACCGTCTCCTCTTACGCCGAGTTCACTGCCGCCGTCCAAGGCGACGACC CCAAAATCGTCATCGTCAGCGGTCCCATCGAGGAGACCGCCGAACAGGTC GATGTCGGCTCTAACACCTCTATCCTTGGTGCCGACTCTTCCGCCGTCCT GACCGGTTTCGGTCTCCGCCTCAAGGAGGTCGAGAACGTTATTATCCGCA ACCTCGGTATCGCGAAGGTCTTGGCCGACAACGGCGACGCCATCGGTGCT GAATACTCCAACAACATCTGGATCGACCACGTCGACGTCTCCAGTGACCG TGACCACGACAAGGACTACTACGACGGTCTCCTTGACTTCAAGCGCGGCT CTGACTACATCACCGTCTCTAACTCCTTTATTCACGACCACTGGAAGGCT TCGCTCGTCGGCCACAGCAACAGCAACGAAGACGAGGACTCTGGAAAGCT CCACGTTACCTACGCTAACAACTACTGGTACAACCTGAACTCCCGCGCTC CGTCAATCCGCTTCGGTACCGGCCACATCTACAACAACTACTACGAAACT GTGAGCGACGGCATCAACACCCGTATCGGTGCCCAGGTTCTCGTCGAGGG TAACGTCTTCGTCGACAGCAAGAAGGCCCTCTACTCGACTGACGAAGGAT ACGCCGTCGAGCGCAACAACGACTTTGGTGATGCCAAGAACGAGGCCCTT GAGGGAACCCTTACCTCCGTTGAATACGAGTACGACCTCCTTGATACCTC TGAGGTCAAGAGCGCTGTTGTTGGAACTGCTGGTCAGACCCTTACTTTTC TAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCAT CATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMQNLKFLIAAVSCLGPALAAPTPTSNFINSNFNKRASVEDSAFG YASLNGGTTGGAGGTTTTVSSYAEFTAAVQGDDPKIVIVSGPIEETAEQV DVGSNTSILGADSSAVLTGFGLRLKEVENVIIRNLGIAKVLADNGDAIGA EYSNNIWIDHVDVSSDRDHDKDYYDGLLDFKRGSDYITVSNSFIHDHWKA SLVGHSNSNEDEDSGKLHVTYANNYWYNLNSRAPSIRFGTGHIYNNYYET VSDGINTRIGAQVLVEGNVFVDSKKALYSTDEGYAVERNNDFGDAKNEAL EGTLTSVEYEYDLLDTSEVKSAVVGTAGQTLTFLEQKLISEEDLNSAVDH HHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGCAGAACCTCAAGTTCCTC (Pml I) TACAGTCTAGAAAAGTAAGGGTCTGACCAGC (Xba I) |
| pH optimum: | 8.5 |
| Specific activity (Substrate): | pectic acid |
| T optimum: | 22 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on pectic acid, Ca2+ is cofactor, produces GalA3-5 with unsaturated GalA at the NR end |
| References: | not yet available |
pectate lyase