Enzyme Function:alpha-galactosidase
Accession number: AN7624.2
GenBank ID: DQ490512
FGSC number: 10117
Average molecular weight
(clone, calculated): 		53.719 kDa
AA length (clone): 483 aa
DNA length (clone): 1452 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGATCGAGTTTCTAGCACTGATCACCCTGAT TTCCCGAGCCAACGCGCTCATGAGGCCTGACGGAGTGGGCCGCCTCCCAG CTCTAGGTTGGAGCTCTTGGAATGCGCACGAGTGTGACATCAACGCAACT GTAATTCTGACTGCCGCAGCGCAAGTCGTGAAGTTAGGACTCAAGGATTT GGGCTATGAATATATCAACATCGACGACTGCTGGTCCATAAAAACCCACC GCGACCCCACCACAAACCGAATGATCCCAGACGCAGATCGATTTCCTGAC GGAATCGCCAGCGTAGCATCGCAGATCCATGAGCTAGGCCTGAAAGTTGG AATATACAGCAGCGCGGGTGAAACAACGTGCGCCGGGTACCCAGCTAGTC TGGGGTATGAGGATATCGATGCGGAGACGTTTGCTGAGTGGGAGATTGAC TACCTGAAATACGACGACTGCGGGGTCCCAGATAACTGGAAAGACCCATA CACTTTCTGCGTCCCCGACACAGCCAACAACGCCGGGCCTTTTCCGAACG GCACTTGTCCCAGCCTACCCAATCCCGCACCGGCAAACTACAACTGGAGC ACATCCCCCAGCGCCGAACGCTTTCGCCGCATGCTTGATGCCCTCAATAC CCAGGACCGAACAATTCTCTACTCCCTCTGCAACTGGGGCAACGCAGCAG TGAACACCTGGGGCGCTGAGATTGGGAACTCATGGCGCATGAGCGGGGAT ATCTCTCCGGGGCGCGGGGAAGTAGGGCCCGACAGAACCAGGATTGCAGT CTGGGAGAGAATTGCGGAGATTACGAATGAGATGAGTTTTCTTGTTAGAG AGTATGCGGAGTTTTGGGGATGGCCTGATGCAGATATGCTTGAGGTGGGG AACGGCGAAGGGGGGATGACGGTGGCGGAGAACAGAGCGCATTTTGCGCT TTGGGCGGCGATGAGGAGCCCCCTTCTGATTGGGACGAAACTTGATACAA TCAGACAAGAGCACCTGAAAATTCTTAAGAACCCTACTCTCCTAACATTC CATCAAGACCCCATCATCAACCGCCCGGCTTACCCCTACAAATGGGGTTA CAATGCAGACTACACTTTCGACGCCGCCCATCCAGCAGAATACTGGTCTG GACCCTCGCCTGCGCTAGAAGGCACGTTGGTCGTCATGCTCAACTCCGAG AATGTCACGAGCACGCGCATGGCTGTTTGGAGCGAGGTCCCGGAGTTACA GAATCAAGACCAAGGAGATGCGTTCGAGGTCATGGATGGGTGGACAGGCG AGGATCTTGGTTGCATTAAAGAGAAGTATGAGGTCGAGCTTGACGCGCAC GATGCGGCGGTCTTGGTTGTTGGGAATGCTTGTCTAGAACAAAAACTCAT CTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATT GA
Amino Acid Sequence (clone): SMNSRDMIEFLALITLISRANALMRPDGVGRLPALGWSSWNAHECDINAT VILTAAAQVVKLGLKDLGYEYINIDDCWSIKTHRDPTTNRMIPDADRFPD GIASVASQIHELGLKVGIYSSAGETTCAGYPASLGYEDIDAETFAEWEID YLKYDDCGVPDNWKDPYTFCVPDTANNAGPFPNGTCPSLPNPAPANYNWS TSPSAERFRRMLDALNTQDRTILYSLCNWGNAAVNTWGAEIGNSWRMSGD ISPGRGEVGPDRTRIAVWERIAEITNEMSFLVREYAEFWGWPDADMLEVG NGEGGMTVAENRAHFALWAAMRSPLLIGTKLDTIRQEHLKILKNPTLLTF HQDPIINRPAYPYKWGYNADYTFDAAHPAEYWSGPSPALEGTLVVMLNSE NVTSTRMAVWSEVPELQNQDQGDAFEVMDGWTGEDLGCIKEKYEVELDAH DAAVLVVGNACLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGATCGAGTTTCTAGCACTG (Pml I) TACAGTCTAGACAAGCATTCCCAACAACC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: not active on PNP-alpha-galactoside, raffinose
References: not yet available