Enzyme Function:alpha-xylosidase
Accession number: AN7505.2
GenBank ID: DQ490509
FGSC number: 10114
Average molecular weight
(clone, calculated): 		91.276 kDa
AA length (clone): 808 aa
DNA length (clone): 2427 bp
DNA source: gum arabic cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAAGTTCACGGAGGGCATGTGGCTCCTGCG GGAGGGCATCCGGATCGATTGGATGAGCAACGTCGAGCGATTGAATGTGG ATAAGGACACTGTGAATCTGCTGCTCAATAAGTTCCAGCGACACCGTGGA GATACGCTTAATTCATCTACTGTCTCCGCACGAGTTACTTCTCCCTTAGA GGGTATTATTGGTGTTAAGCTTGTCCATTGGGCCGGCGGCCTAGACAACG GACCACATTACGAACTCAACACCTCCGCGGGCCATACCGAGATCACGCAC GAGAAGGGCAAAAATTTAAAGTATACCAGCGGACGATTGGAATTGGACAT CAACATAGCTCCCAACGAACTAGCCTTCACCTTCACCACTGGCGCAGATG GCCAGGACAAGAGAAAGAAACTTACAGGCCACTCCTTCCGCTCAATCGGA TACGTCGGCGATTCCACAACCCCGAAGTCCCAGCTTAGTGACGGTATCTT CTACGAGCGACAAGGGTACACCCTTGCGGAACTTGATCTGAGCGTTGGCG AAAAGCTCTATGGTCTAGGCGAACGATTTGGTCCTTTCGTGAAAAACGGC CAGAGCGTGAATATATGGAACGAGGACGGCGGAACATCTTCTGAGCTCGC GTACAAGAATATTCCGTTTTATATCAGCTCGAACGGGTATGGAGTCTTTG TCAATCATCCTGGCAAGGTCAGTCTTGAGTTGCAGTCTGAGCGCACAACG AGGGTGAACGTTTCGGTTGAGGGGGAGGAGCTAGAGTATTTTGTTATTGA AGGGAAGAACCCGAAGGAAATCTTGAAGCGGTGGACCGACCTTACAGGAA AGCCGGCGCTCGTCCCCGCGTGGAGTTATGGGTTATGGCTTACCACAAGC TTCACAACAAACTACTCCGAGCGAACCGTCACCGGCTTCCTAGACGGCTT CAAGGACCGGAATCTGCCGCTCAGCGTCTTTCATTTTGACTGCTTCTGGA TGAAGTCCTACCAATGGTGCGACTTCGAGTTTGACGCCGATATGTTCCCC GATGCGGCCGGGTACCTAGCGCGTTTGAAAGAGCGCGGTCTGAAACTCAG TATCTGGATTAATCCGTATGTCGGACAGGCGTCGCCGCTGTTTGAGATTG GAAAGAGGGAGGGGTATTTTATTAAGCGCATCGACGGCTCAGTCTGGCAA TGGGATCTTTGGCAAGCTGGAATGGCTGTCGTTGACTTCACAAACCCGGC CGCATGTTCCTGGTACACCGGTCACCTGAAGCGCCTAATGGATCTCGGAA TTGATACCTTCAAAACCGACTTCGCAGAGCGCATTCCATTCAAGAACATC ACGTACCACGATGGCTCTGATCCCGCCCGCATGCACAACTACTATGCACT TCTATACAACAAAGTCGTCTATGAGACCATGACGTCCATCTCCGGCAAAT CCAACAGCCTTCTCTTCGCACGGTCTACCTCCGTCGGTGGGCAGAAATAC CCCGTTCATTGGGGTGGAGACTGCGAATCCACTTACGAAGCCATGGCCGA GTCCCTTCGTGGTGGGCTTTCTCTGGGACTAGCGGGGTATATATTCTGGG CATCGGATATCGGTGGGTTTGAGGGGACGCCCCCGCCAGCGCTATATAAG CGGTGGGTACAGTTCGGGCTTTTGTCTTCCCACTCACGTCTCCACGGTAG CTCATCATTTCGTGTTCCCTGGATTTATGGGGAAGACTGCTCGGATGTGC TGCGGGACTGCGTGAAGCGCAAAATCAGCTTGACGCCATATCTCCTTGCT GAAGCGTTGAACGGACATAGATCCGGAACACCGCTAATGAGGCCCATGTT CATGGAATTCCCGGAAGATCTGAACACGTACCCGCTTGACACGCAGTATA TGTTTGGCTCGAATTTACTGGTTGCGCCAGTGTTTTCGGACGAGGGAATC GTTACGTTTTATGTTCCAAGAACGCCGGAGGAGGAAGGAAGAAAGCAGTG GATCTCCTGGTTCGACCACGGCAAGAAATACGAAGGTGGACGGTGGTATA CAGAGACACACGGTTTCGATACCCTGCCAATCCTCATTCGTCCTGGCTCT GTGACGCCCATTAACTACAAGCTTGAGAAGCCGGAGGGAAATCCGTTGGA TGGGCTGGAGATCTTGGTGAATGGATCTATCGATAAGGAGGTGGAAATTG AGATTGTGGATCCCGAGACAACGCATAAGGTTTTGAAGGTTATGACTGTT TCGGAGAGAGAGACAGAGAACGGAGTTGAAGTGATTGCAAGACTTGATGG GGTTGATGGTAATGAGAACAGCGTGAAAGTAAATTGGGTTGGGCATGGAG TAACTAATCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCC GTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMKFTEGMWLLREGIRIDWMSNVERLNVDKDTVNLLLNKFQRHRG DTLNSSTVSARVTSPLEGIIGVKLVHWAGGLDNGPHYELNTSAGHTEITH EKGKNLKYTSGRLELDINIAPNELAFTFTTGADGQDKRKKLTGHSFRSIG YVGDSTTPKSQLSDGIFYERQGYTLAELDLSVGEKLYGLGERFGPFVKNG QSVNIWNEDGGTSSELAYKNIPFYISSNGYGVFVNHPGKVSLELQSERTT RVNVSVEGEELEYFVIEGKNPKEILKRWTDLTGKPALVPAWSYGLWLTTS FTTNYSERTVTGFLDGFKDRNLPLSVFHFDCFWMKSYQWCDFEFDADMFP DAAGYLARLKERGLKLSIWINPYVGQASPLFEIGKREGYFIKRIDGSVWQ WDLWQAGMAVVDFTNPAACSWYTGHLKRLMDLGIDTFKTDFAERIPFKNI TYHDGSDPARMHNYYALLYNKVVYETMTSISGKSNSLLFARSTSVGGQKY PVHWGGDCESTYEAMAESLRGGLSLGLAGYIFWASDIGGFEGTPPPALYK RWVQFGLLSSHSRLHGSSSFRVPWIYGEDCSDVLRDCVKRKISLTPYLLA EALNGHRSGTPLMRPMFMEFPEDLNTYPLDTQYMFGSNLLVAPVFSDEGI VTFYVPRTPEEEGRKQWISWFDHGKKYEGGRWYTETHGFDTLPILIRPGS VTPINYKLEKPEGNPLDGLEILVNGSIDKEVEIEIVDPETTHKVLKVMTV SERETENGVEVIARLDGVDGNENSVKVNWVGHGVTNLEQKLISEEDLNSA VDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAAGTTCACGGAGGGC (Pml I) TACAGTCTAGATTAGTTACTCCATGCCCAACC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-alpha-xyloside
References: not yet available