Enzyme Function:alpha-1,3-glucanase
Accession number: AN7349.2
GenBank ID: DQ490508
FGSC number: 10112
Average molecular weight
(clone, calculated): 		51.370 kDa
AA length (clone): 459 aa
DNA length (clone): 1380 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAAGATCTTCCACCGCTGCTGCGCAGCCCT AACTCTTCTAGCCAATGCTCTCCCGGCTCTATCGCTACCGGGAGCGAACA GTCTCACTATTCGCAAAGACTCGAACAAATATGTCACGGCGCATTTCATG GTTGGCATCGTCGAAAACTATACCGTCGACGACTGGAAGCACGATATGGA GCTAGCCAAGGAAACGGGAATCGACGCATTCGCTCTTAACTGCGCCAGCA TCGACTCCTACACAGACAAGCAGCTGGCATATGCGTACGAGGCAGCTGAA GAGGTCGACTTTAAGGTGTTTATCTCGTTCGACTTCGCATATTGGTCGAA CGGCGACACTGCCAGAATTACCTCCATTATGCAGACCTATGCCGACCATC CAGGCCAGTTTCAATATAATGGTGCTGCGTTAGTGAGCACGTTCGTCGGG GACAGTTTCGACTGGGGTCCAGTCAAGAGGGCAGTAGATCATCCAATCTT TGCGGTTCCGAATCTGCAGGATCCGAACTGGGCCGGCCACGCGACGACAT CGATTGATGGAGCGTTTTCGTGGTATGCGTGGCCGACGGATGGAGGAAAT AGTATTATCAAGGGCCCCATGACGACAATTTGGGATGATAGGTTCAGGAA TAATCTCAAGGACAAGGTTTATATGGCTCCTGTCTCGCCGTGGTTTTCAA CCCACTTCAACACCAAGAACTGGGTATTTATCTGTGAAGACCTTCCACAT CTCCGCTGGCAGCAGATGCTGGAAATGCAGCCAGAATTGATCGAGATCAT TTCCTGGAATGACTACGGCGAGTCCCACTACATCGGCCCTTACTCAGAAG CCCACTCTGACGACGGCTCTGCGCAATGGACGAAAGACTTTCCCCACGAC GCATGGCGCATCATCGCCAAGCCCTACATCGCCGCATACAAGGCGGGAGA GAGAGAGCCCACTGTGGAGTCTGACCAGCTGGTCTACTGGTACAGACCGA CACCGAAAGCCGTTACCTGCTCGAAGGATCCGCTTGGTCCGCCAAATGGA ATCAACCTGCTTGAGGATAGTGTCTTTGTTACGACGCTGCTCACCGAGCC GGCCACATTGACTGTCGGCAGCGGCTCCCTGGAGTTTTCGGTTGACGTAG ATGCTGGCATTGTGACAAACAGCTTCCCCATGGGTGTCGGCTCGCAGGCC TTTTCTGTCACCCGCGACGGCGAGGAGATCCTCGGCGGCGATGGAGGGCT GGATGTTCAGGATAGATGTGACTATTATAACTTCAATGTTTATGTTGGCT CTTTTAGCGCTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGC GCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMKIFHRCCAALTLLANALPALSLPGANSLTIRKDSNKYVTAHFM VGIVENYTVDDWKHDMELAKETGIDAFALNCASIDSYTDKQLAYAYEAAE EVDFKVFISFDFAYWSNGDTARITSIMQTYADHPGQFQYNGAALVSTFVG DSFDWGPVKRAVDHPIFAVPNLQDPNWAGHATTSIDGAFSWYAWPTDGGN SIIKGPMTTIWDDRFRNNLKDKVYMAPVSPWFSTHFNTKNWVFICEDLPH LRWQQMLEMQPELIEIISWNDYGESHYIGPYSEAHSDDGSAQWTKDFPHD AWRIIAKPYIAAYKAGEREPTVESDQLVYWYRPTPKAVTCSKDPLGPPNG INLLEDSVFVTTLLTEPATLTVGSGSLEFSVDVDAGIVTNSFPMGVGSQA FSVTRDGEEILGGDGGLDVQDRCDYYNFNVYVGSFSALEQKLISEEDLNS AVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAAGATCTTCCACCGCT (Pml I) TACAGTCTAGAGCGCTAAAAGAGCCAACA (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on alpha-1,3 glucan (mutan) from A. nidulans
References: not yet available