Enzyme Function:rhamnogalacturonan lyase
Accession number: AN6395.2
GenBank ID: DQ490501
FGSC number: 10105
Average molecular weight
(clone, calculated): 		77.265 kDa
AA length (clone): 688 aa
DNA length (clone): 2067 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGCGTCTCGGTGTCTGTTTCAGTCTTGCTGC GGCGGCCAGTGTCGCACGCGCAGCCCTAACTGCAACAGAGAATGACACCT CCATTGTCCTTGAGAACGACCGGCTCAGAGCCACCTTCGACAAAGGCAGG GGGAGTATCATCGACCTCTACCTTGACGGTCAAGATTTCCTAGGTCCGCA GTCTGGATCGACCGGGATCGGCCCCTATCTCGACTGCTACTGCACCCCCT CCGGTTTCTACACCGCTGGTTCAACAAATCCTGTAACAGAGCTGGTGCAA GGCACAGACTCGACCGGCACAAAATATGCAGGCATCATCCTGAACGATAC GTATACCCCGACCGGCCAAGAGTTCCAGCAGTACTGGTTCCTCCGCGATG GCGAGACCGGTTTCCATATGTTCAGTCGATTGGCGTACTACAACGAGACC ACCCCGTTCCTGCGCAATCTACAAGAGCTCCGCACGCTCTTTCGCCCGAA TACGGATCTATGGACACATCTCACCTCGAGTGACCTGCAGACTGCGCCCT TGCCCAGCGACGAGGCTATTGCGGAGCAGATCGTTGTCCAGGACGCGACC TGGAGGCTTAATAACACGCCGGACGATGCATACTACCAGCAGTTTTCAGA GTACTTTACCAAGTACACCTTCTCGAACCACTGGCGAGACAATGATGTCC ACGGACTCTACGCGGACGGGTCTACCTCGGATGGGACTACATACGGCGCA TGGCTGGTGATGAATACAAAGGATACCTACTACGGACTGACAGAGCAGGG CCCGCTGCACTCTGACCTCACCGTCGACGGTATAATCTACAACTACATCG TTTCCAACCACCACGGCGAAGGCACTCCGAACATAACCAACGGCTTTGAC CGCACCTTCGGACCGCAGTTCTACCTCTTCAACGGCGGAGGATCCTCCTC ACTAGAGGAGCTCAGAGACGAGGCTCGCTCTCTAGCGAGCCCAAGCTGGA ACGCCGACTTCTACGACTCGATCGCCAAACACGTCATTGGTTACGTCCCT TCGTCGCAGCGCGGCAGCGTAAAAGGCACGATCAAGCTGCCCAAGAACGC GAAAAGTCCCATCGCCGTCCTCACCGTCGACGGCCACTACTTCCAGGATA ACTCTGCCGTTCCCTCGTCCCACCAATACTGGGCCGACATCGACAAGAAT GGCCGCTTCAGCATCGACCGCGTCGTTGCCGGCAAATACCGACTAACCGT CTATGCAGACGGCATTTTCGGCGACTTCACACGCGACGGGATCGTTGTCA AAGCACGCAAATCAACATCCATCAAGGAGACATGGAAGCCCGAATCTGCT GGCACAGAGATCTGGCGTCTCGGTACCCCCGACAAATCCTCCGGCGAGTT CCGCCACGGCGCGGCGCGCGATCCTACCCATCCCCGCCACCCGCCAGAGT ACCTGATCTACTGGGGTGCCTACGACTGGCAATCTGACTTCCCCGGCGGG ATTGACTATATGATCGGTGAGAGCGATCCAGCAACAGACTTCAATACAGT CCACTGGGCTGTCTTCGGACCCACGCCCGACAACCCCGTAGCGGAATCGA ACACCACCCACGACTGGAGAATCCGATTTGATCTAAGCGCGAAACAGCTA CACGCACGTAAGACGGCCACACTAACGATCCAGCTTGCGGGTGCAAAGGC TGCGAGCGGAAACACGGACGTGTATAACGCGTCCGAGCCGTACGCGAATC TCCCGCTGCGGAGCTACATCAATGAGCAAGAGGAGCCTTTGACTATGGTT ATTGGCTATGATCAATCGAGTAGCTGTATTGTGCGGTCGGCAGTTAGTTG CTACCAGGTGCGTGAGAAATGGGAGTTCCCAGCTAGTTGGTTAAAGGAGG GAAGTAATTTACTGAGGCTGAGCTTGCCGACTAATGGGACGAATTACGAG AGTGCGGTGTTGCCGACGAGCGTGTACGTGCAGTATGATGCTTTGAGATT GGAGTTGAATCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCG CCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMRLGVCFSLAAAASVARAALTATENDTSIVLENDRLRATFDKGR GSIIDLYLDGQDFLGPQSGSTGIGPYLDCYCTPSGFYTAGSTNPVTELVQ GTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRDGETGFHMFSRLAYYNET TPFLRNLQELRTLFRPNTDLWTHLTSSDLQTAPLPSDEAIAEQIVVQDAT WRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGA WLVMNTKDTYYGLTEQGPLHSDLTVDGIIYNYIVSNHHGEGTPNITNGFD RTFGPQFYLFNGGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVP SSQRGSVKGTIKLPKNAKSPIAVLTVDGHYFQDNSAVPSSHQYWADIDKN GRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSIKETWKPESA GTEIWRLGTPDKSSGEFRHGAARDPTHPRHPPEYLIYWGAYDWQSDFPGG IDYMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQL HARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEPLTMV IGYDQSSSCIVRSAVSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYE SAVLPTSVYVQYDALRLELNLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGCGTCTCGGTGTCTGTT (Pml I) TACAGTCTAGATTCAACTCCAATCTCAAAGCA (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on linseed RG produces (GR)n oligomers with unsaturated GalA at the NR end 1 silent mutation in Pichia clone: nucleotide 1414 is G instead of A
References: not yet available