Enzyme Function:ferulic acid esterase
Accession number: AN5267.2
GenBank ID: XM_657779
FGSC number: 10099
Average molecular weight
(clone, calculated): 		31.232 kDa
AA length (clone): 298 aa
DNA length (clone): 897 bp
DNA source: genomic DNA
DNA Sequence (clone): TCGATGAATTCACGTGCAATGCTTCGAGCCGTCCTCCTCCCTACTCTTCT GGCCTTCGGCGCCTTCACCCCCGTCCATGGCGCCAATTCGCCAGGCTGCG GTAAACAGCCGACCCTCACCAATGGCGTCAACCAAATAAACGGCCGCGAG TACGTTCTCAAAATTCCCGATGGCTACGACCCAAGCAAGCCACATCATCT CATCTTCGGCCTTCACTGGCGCGGCGGGAACATGTACAATGTCGTGAACG GCGACAGCATCCAGCCATGGTACGGCCTCGAGGCCCGCGCGCAAGGGAGC GCGATCTTTGTAGCCCCCAACGGTCTGAATGCTGGCTGGGCCAACACCAA CGGCGAGGACGTGGCCTTCATCGACGCCATCATGGAGCAGGTTGAAGACG ATCTCTGCGTGGACCAGGCCTCTCGCTTCGCGACAGGGTTCTCCTGGGGC GGAGGTATGAGCTATGCCCTCGCTTGCGCGCGGGCTGCTGAGTTCAGAGC CGTGAGCGTACTGAGCGGTGGCTTGATCAGCGGATGTGACGGGGGCAACG ACCCCATTGCATATTTGGGGATCCACGGAATCAATGATCCTGTTCTTCCG CTTGACGGAGGTGTGACGCTTGCAAATACCTTTGTGTCGAATAATGGTTG CCAGCCCACGGATATCGGTCAGCCTGCGTCTGGCAGTGGCGGGTCCGTTC GAACGGACTTCAGTGGCTGCTCTCACCCGGTTTCGTTTATTGCATATGAT GGCGGGCATGATGGGGCGCCGCTTGGTGTTGGAAGCTCGCTTGCTCCGGA TGCAACCTGGGAGTTTTTCATGGCGGCTCTAGAACAAAAACTCATCTCAG AAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRAMLRAVLLPTLLAFGAFTPVHGANSPGCGKQPTLTNGVNQINGRE YVLKIPDGYDPSKPHHLIFGLHWRGGNMYNVVNGDSIQPWYGLEARAQGS AIFVAPNGLNAGWANTNGEDVAFIDAIMEQVEDDLCVDQASRFATGFSWG GGMSYALACARAAEFRAVSVLSGGLISGCDGGNDPIAYLGIHGINDPVLP LDGGVTLANTFVSNNGCQPTDIGQPASGSGGSVRTDFSGCSHPVSFIAYD GGHDGAPLGVGSSLAPDATWEFFMAALEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): CATTTCACGTGCAATGCTTCGAGCCGTC (Pml I) GCTTGTCTAGAGCCGCCATGAAAAACTCC (Xba I)
pH optimum: 6.1
Specific activity (Substrate): methyl ferulate
T optimum: 37
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-acetate, methyl ferulate, and removes FA from the oligomers released from insoluble wheat arabinoxylan
References: not yet available