Enzyme Function:endo-polygalacturonase
Accession number: AN4372.2
GenBank ID: DQ490492
FGSC number: 10094
Average molecular weight
(clone, calculated):
40.594 kDa
AA length (clone): 387 aa
DNA length (clone): 1164 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCTATGCATTTCCTTCAAAACTCCCTTATTGCTGCGGCAAT GGGCGCTGCGCTGGTCGCTGCCGCCCCTGCTGCTGATCTTGATGCTCGAA GCTCGTGCACCTTCACCTCTGCCTCTGCGGCCAAGTCTGGCGCATCCAAG TGCTCCACTGTCACCCTCAAGAGCATCCAAGTTCCTGCCGGTGAGACCCT TGACCTGACCGGTCTCAAATCGGGTGCTACTGTACGCGAGACAACCTTTG GCTACAAAGAATGGAAAGGACCGCTGATCTCCATGTCCGGTGACAAAATC ACGGTTAAGCAAGCCTCTGGCGCAAAGATCAACTGCGACGGGGCCCGCTG GTGGGACACCAAGGGCAGCAACGGCGGCAAGACCAAGCCCAAGTTCTTCA GCGCGCATAAGCTGAACAACTCCAAGATTCAGGGGCTGAAGATCTACAAC ACCCCTGTCCAGGGATTCAGTATCCAGTCCGACCACCTGACCATTTCGGA CGTGACCATCGACAACTCCGCCGGCACCAGCAAGGGCCACAACACCGATG CCTTTGACATCGGCTCCTCGACGTACATTACCATCGACGGTGCGACTGTC TACAACCAGGATGACTGTATTGCCATTAACTCCGGCGAGCACATCACCTT CACCAACGGATACTGTTCCGGCGGCCATGGCTTGTCTATTGGCTCCGTCG GCGGCCGCAGCGACAACACCGTCAAGAGCGTCACCATCTCCAACAGCAAG GTCGTCGACTCCCAAAACGGCGTCCGCATCAAGACCGTCTACAAGGCTAC CGGCTCCGTCACCGATGTCACCTTCCAGGACATCGAACTCTCTGGAATCA CCAAGTACGGCCTCATTGTTGAGCAGGACTATGAGAATGGTAGCCCAACA GGTACCCCTACCAACGGTGTCGAGGTTGAAGATATCACCTTCAAGAAGAT TACCGGCTCTGTGGATAGCAGTGCCACACGTGTCAATATCCTCTGCGGGT CAGGGAGCTGCAAAGACTGGACTTGGTCTGGGGTTGATATTACCGGCGGA AAGAAGAGCTCTAAGTGCAAGAATGTTCCGTCTGGTGCTTCGTGTCTAGA ACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATC ATCATCATCATTGA
Amino Acid Sequence (clone): SMNSMHFLQNSLIAAAMGAALVAAAPAADLDARSSCTFTSASAAKSGASK CSTVTLKSIQVPAGETLDLTGLKSGATVRETTFGYKEWKGPLISMSGDKI TVKQASGAKINCDGARWWDTKGSNGGKTKPKFFSAHKLNNSKIQGLKIYN TPVQGFSIQSDHLTISDVTIDNSAGTSKGHNTDAFDIGSSTYITIDGATV YNQDDCIAINSGEHITFTNGYCSGGHGLSIGSVGGRSDNTVKSVTISNSK VVDSQNGVRIKTVYKATGSVTDVTFQDIELSGITKYGLIVEQDYENGSPT GTPTNGVEVEDITFKKITGSVDSSATRVNILCGSGSCKDWTWSGVDITGG KKSSKCKNVPSGASCLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGGAATTCTATGCATTTCCTTCAAAACTC (EcoR I) TACAGTCTAGACACGAAGCACCAGACGG (Xba I)
pH optimum: 5.1
Specific activity (Substrate): pectic acid
T optimum: 38
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on pectic acid, less active on citrus pectin, produces GalA1-3 with slow conversion to GalA1 and GalA2
References: not yet available