Enzyme Function:beta-mannosidase
Accession number: AN3368.2
GenBank ID: DQ490488
FGSC number: 10089
Average molecular weight
(clone, calculated): 		99.840 kDa
AA length (clone): 875 aa
DNA length (clone): 2628 bp
DNA source: gum arabic cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGGGTGCCTTCACACAACACGTTCTTTCAGA GGGTTGGAGCTTTAAGGACAGCGGTGATCAGTCCCCAGATGCCTGGTTGT CGGTTCCCACTGTTCCTTCTGTCGTCCATCAAGACTTACAGGCAAATGGC AAATTAGATGATCCGTTCATTGGCCTCAACGAATTGAGTGCAAGATGGGT CAACGAGAAGTCATGGACCTACCGAAACGTCTTCCAGAAGCCAACCGTTC CTGCTGGATCTTCAATCTTTTTAGTCTTTGACGGACTGGATACTTTCGCC AAGGTCAAACTCGATGGCCAGGTCATTCTAGAGAGCGATAACATGTTTCT TGCTCACCGGGTGGATATCACAAAAGCCCTAGATGTGGAAGGCGAGCATA CACTCGAGATTGATTTCGATTGTGCCTTACTTCGTGCTCGAGAACTTCGA AAGCAGCACCCAGATCATAAATGGGTTGGTTTCAATGGAGACACTGCGAG ATTGAGCGTCAGAAAAGCTCAGTATCATTGGGGTTGGGACTGGGGGCCAG TCCTGATGACAGCAGGCATTTGGAAAGAAGTGCGACTTGAAGTTTATTCC GCGAAAATTTCAGATCTCTGGACAGAGGTGCACCTAGCCGAGGACCACTC CAAGGCTCGCATTACCGCTGCCGCAGAGGTTGAGACCCAAGGGACCGGCA ACTCGTACAAAGCTACTTTTACGTTGAGCCTTCAAGGGCAGCAGATCGGC AAAGAGGTAGCTACTCTGGACGGCAATGTCGCTAAGACGACGTTCGATGT TCAAGAACCGTCGCTTTGGTGGCCGAATGGATACGGAGACCAAACTTTAT ATGAGATCTCGGTGTCTCTGGAAAAGGAAGAAGAACAAGCCCATCAGGTC TCGAAAAAGTTTGGCATTCGGACTGCGGAGGTCATCCAGCGGCCGGACAA GCATGGAAAATCCTTCTTCTTCCGCATTAACGGTGTTGATATTTTCTGCG GCGGTGCATGTTGGATTCCCGCTGATAGTCTTCTGACCAATATCACACCC GATCGTTATCGCAAATGGATCGAGTTAATGGCTGTCGGCCATCAGGTGAT GATTCGTGTTTGGGGTGGCGGCATCTACGAGGATGAGAGCTTCTACCAGG CCTGTGATGAGGTCGGTGTAATGGTATGGCAAGATTTTATGTTCGGCTGC GGTAACTATCCGACGTGGCCTGAGATTCTTGAGTCGATCGAAAAAGAGGC CGAATATAACCTGCGGAGATTGCGCCACCACCCTTCCATCGTTATCTGGG TTGGAAACAACGAGGACTATCAGGTACAGGAGCAGCAAGGGCTCACTTAC AATTATGCAGACAAGGATCCGGAGAGTTGGCTCAAGACGGATTTCCCGGC CCGCTATATCTACGAGCATCTTCTACCAAAGGCCGTCCAAAAAATAATTC CATCGGCATATTACCATCCTGGCAGCCCGTGGGGCGATGGCAAAATCACT TCTGACCCGACCGTAGGGGATATGCATCAGTGGAATGTATGGCACGGTAC ACAGGAGAAATACCAAATCTTCGATACTCTTGGCGGCCGCTTTAACAGCG AGTTCGGAATGGAAGCGTTCCCTCACATGTCCACCATCGACCACTTCGTC ACAAATGAAGCCGACAAATACCCCCAGTCACACGTCCTGGATTTCCACAA TAAGGCAGACGGTCACGAGCGGAGGATTGCCACGTACCTCGTTGAGAATC TACGAACGGCAACAGACTTAGAAGTCATCGTCCAAGCAGAAACGATGATG TTTGGTTACCGCGGATGGCGCCGCCAGTGGGGAGATGAGAGACATTGTGG AGGTGCACTGCTCTGGCAATTGAACGACTGCTGGCCAACCATCTCCTGGG CCATTGTAGACTACTTTCTGCGCCCCAAACCTGCGTTCTATGCCGTCAGC CGTGTTTTGAAGCCGCTTGCCATTGGTGTGCGCCGGGAGCACCACGATTG GAGTGTCTCCCACGCACAACCGCCTAAGACTAGCAAGTACGAGCTATGGG TCGTCAGCAGTCTGCTGAAGGAGGTGATCGGAAAGGTCGAACTTAGGTTT ATATCCATCAAGACTGGTCTTGCTATTCACGAGTCTATCGTCCGCGAGAA TGTCACCATTGTACCCAATGGCACTACTAACATCTTGGACGGAGTCATCG ATCACGCAGTCGACGAGCCGCATGTCCTTGCTGCCAGACTCTGGGTGGAT GGCGAGCTTGTGGCTAGAGATGTTGATTGGCCGCAGCCGTTCAAGTACCT TGATCTCTCTGACCGAGGACTAGAGATTACGAGGATTTCCAAGACCGAAA GCGAGCAGGTACTTGAATTGAGCGCCAGGAAGCCAGTCAAATGCTTGGTC TTTGAGGAACGAGACAATGTCCGAGTCAGCGACAGTGCTATTGACATCGT GCCGGGCGATGAGCAGTTTGTTACTATTAAGGGCTTAAAGCGTAGTGATG CGCCGCTGAAGTACAAATTCCTTGGACAGGTACCTCGAGCCGCGGCGGCC GCCAGCTTTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGC CGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMGAFTQHVLSEGWSFKDSGDQSPDAWLSVPTVPSVVHQDLQANG KLDDPFIGLNELSARWVNEKSWTYRNVFQKPTVPAGSSIFLVFDGLDTFA KVKLDGQVILESDNMFLAHRVDITKALDVEGEHTLEIDFDCALLRARELR KQHPDHKWVGFNGDTARLSVRKAQYHWGWDWGPVLMTAGIWKEVRLEVYS AKISDLWTEVHLAEDHSKARITAAAEVETQGTGNSYKATFTLSLQGQQIG KEVATLDGNVAKTTFDVQEPSLWWPNGYGDQTLYEISVSLEKEEEQAHQV SKKFGIRTAEVIQRPDKHGKSFFFRINGVDIFCGGACWIPADSLLTNITP DRYRKWIELMAVGHQVMIRVWGGGIYEDESFYQACDEVGVMVWQDFMFGC GNYPTWPEILESIEKEAEYNLRRLRHHPSIVIWVGNNEDYQVQEQQGLTY NYADKDPESWLKTDFPARYIYEHLLPKAVQKIIPSAYYHPGSPWGDGKIT SDPTVGDMHQWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIDHFV TNEADKYPQSHVLDFHNKADGHERRIATYLVENLRTATDLEVIVQAETMM FGYRGWRRQWGDERHCGGALLWQLNDCWPTISWAIVDYFLRPKPAFYAVS RVLKPLAIGVRREHHDWSVSHAQPPKTSKYELWVVSSLLKEVIGKVELRF ISIKTGLAIHESIVRENVTIVPNGTTNILDGVIDHAVDEPHVLAARLWVD GELVARDVDWPQPFKYLDLSDRGLEITRISKTESEQVLELSARKPVKCLV FEERDNVRVSDSAIDIVPGDEQFVTIKGLKRSDAPLKYKFLGQVPRAAAA ASFLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGGGTGCCTTCACACAAC (Pml I) TACAGGGTACCTGTCCAAGGAATTTGTACTTCA (Kpn I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-beta-mannoside
References: not yet available