| Enzyme Function: | endo-beta-1,4-mannanase |
| Accession number: | AN3358.2 |
| GenBank ID: | DQ490487 |
| FGSC number: | 10088 |
| Average molecular weight (clone, calculated): | 45.074 kDa |
| AA length (clone): | 418 aa |
| DNA length (clone): | 1257 bp |
| DNA source: | xylan cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGAAGTTCTCGCAGGCGTTGCTTTCCCTGGC CAGCCTGGCGCTGGCCGCTGCGCTCCCTCACGCGTCAACACCTGTGTACA CTCCTTCAACTACACCTTCTCCTACTCCTACTCCATCCGCATCCGGATCT TTCGCTACTACTTCGGGCATCCAGTTCGTCATTGATGGGGAAGCAGGTTA CTTCCCAGGCTCCAATGCGTACTGGATCGGGTTCCTGAAGAACAACAGCG ACGTCGATCTCGTCTTCGACCACATGGCCTCATCTGGCCTGCGCATCCTC CGCGTTTGGGGCTTCAACGATGTGAACACCGCTCCCACTGATGGAAGTGT TTACTTCCAGCTACATCAGGACGGAAAGTCGACCATCAATACCGGCAAGG ACGGACTCCAGCGCCTCGACTATGTTGTTCATAGTGCAGAAAAACACGGT ATCAAGCTGATTATCAATTTCGTGAACTACTGGGATGATTATGGTGGCAT GAACGCGTACATGCGGGCTTATGGGGGCGGTGACAAGGCCGATTGGTTTG AGAATGAGGGCATCCAGGCTGCATATCAGGCGTACGTCGAGGCTGTGGTG AAACGATACATTAACTCAACGGCGGTTTTCGCGTGGGAACTCGCGAACGA GCCACGCTGCACCGGCTGTGAGCCGAGTGTGTTGCATAACTGGATTGAGA AGACGAGCGCGTTTATCAAGGGCTTGGATGAGAAGCATTTGGTTTGCATC GGCGACGGCTCCGATGGAAGCTATCCCTTCCAATACACCGAGGGCTCTGA CTTTGCTGCGGCCCTAACCATCGATACCATTGATTTCGGGACGTTCCATC TCTACCCGGATAGCTGGGGCACAAATAACGACTGGGGCAAGCTCTGGATC ACTTCGCACGCCGCTGCCTGCGCCGCCGCTGGAAAACCGTGTCTGTTCGA AGAATATGGCGTGACGTCGAACCACTGCGCCATTGAGAAACAGTGGCAGA ATGCAGCTCTGAACGCGACGGGCATCGCGGCCGACCTTTACTGGCAGTAT GGGGATACCCTGAGCAGCGGTCCCTCTCCAGACGACGGGAACACGTTCTA TTACGGCAGTGAAGAGTTTGAGTGTCTTGTCACAAACCATGTCGAGACGA TTGAGAGGAGTGCCAATCTAGAACAAAAACTCATCTCAGAAGAGGATCTG AATAGCGCCGTCGACCATCATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMKFSQALLSLASLALAAALPHASTPVYTPSTTPSPTPTPSASGS FATTSGIQFVIDGEAGYFPGSNAYWIGFLKNNSDVDLVFDHMASSGLRIL RVWGFNDVNTAPTDGSVYFQLHQDGKSTINTGKDGLQRLDYVVHSAEKHG IKLIINFVNYWDDYGGMNAYMRAYGGGDKADWFENEGIQAAYQAYVEAVV KRYINSTAVFAWELANEPRCTGCEPSVLHNWIEKTSAFIKGLDEKHLVCI GDGSDGSYPFQYTEGSDFAAALTIDTIDFGTFHLYPDSWGTNNDWGKLWI TSHAAACAAAGKPCLFEEYGVTSNHCAIEKQWQNAALNATGIAADLYWQY GDTLSSGPSPDDGNTFYYGSEEFECLVTNHVETIERSANLEQKLISEEDL NSAVDHHHHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGAAGTTCTCGCAGGCG (Pml I) TACAGTCTAGATTGGCACTCCTCTCAATCG (Xba I) |
| pH optimum: | 5.5 |
| Specific activity (Substrate): | LBG |
| T optimum: | 52 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on LBG, GG, releases equal amounts of Man1 and Man2 from Man5 enzyme has transglycosylation activity: after incubation with Man5 oligos up to Man10 are observed |
| References: | not yet available |
endo-beta-1,4-mannanase