Enzyme Function:acetyl xylan esterase
Accession number: AN3294.2
GenBank ID: DQ490484
FGSC number: 10085
Average molecular weight
(clone, calculated): 		47.714
AA length (clone): 430 aa
DNA length (clone): 1293 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGATGTGGTTTCGCTTTCTGCTTGTGGCCCT GCTCTGGTCGCTCGGCTCGGCCACGATCATGGAGAATGGCCAGCCCCGAG CCGATCCTTACCCGGGCCAGTGTTCAACTATCGATCTGGATAGCAGCTGG AGGAGTTACGATGCCGATGCACCTGAGATCTCGTACAAGGGAAGAGCTCC GGGGCTCAAGTTACAGTTCTCAGGTAAAAAGCTTGCGCTGAGTTTCGGTG AACACACCAGCGAAGGAACGCTCGTCGCTTACCGAATCGGAACGCTGGAT TGGCTCTTCACAAACGTTACCGCAGATGCGACGTATCAGTTCGTCGGGGA GGGAACACACTACGAGGAGTTACCCGGTGATGGAGACCACATTTTTGAGA TGAGAGTCCAGATTGCTGGGGTGTCCGTTGATGTCGATGACCACCTCACG AAACCTCCGACTTTCAAGAAGAAAGTTGAAATTATTGGAGGATCTGCCAG TTTGACCGGCGGTCAGTATGCGACATACGAGACGCTTTCAGCATGGTCGT TTGTATACGCGCAAGGGCTAGGAAATGTCGAATTTGGGATCACAGCCTAC CCTGGAGCATGCCTGGTTGACATGCAGTGCTACGGTGGCGGCGTGCACGG AATGATCTGGTATTGGCACCGTGCCTCCGACCCCGGTTCCCGCGCGCGCA ACATGTATGGAGGCGAGCCCGAAGAATGGGACGTCACCGCTGAACAACCG GCCGACCTGGTCATCATCCAAATGGGCGGTAACGACCACCGGCATCCGAA CGAGATCCCAGGAGAGAACTTTTACCAGGGATACGTTGATCTCATTGAGA ATATCCATCAGCATTGGCCCGACGCGTCGATTGTGATTATGACACAATGG GGCATCTGGAACCAAGAAGGCAGCGAATATACTCAGTCAGTGATCTACGA AGAAGAGATCCAGCGCGTCTACGAGCATTTCAAAGACCGCAAATGGCGCT TCGTCTACCTGTTCCACACGGACGGCATTCTCGGCCACAACGATATCAAC CCAAAGAACCATCCCACGGATGTTGGACACATCAAGATTGCAAGCCATCT GCTCCAATGGACGAATCTGATGCTGGGGTGGGAGTTGAATCCGCGCGGGG AGATGCAGCACGGCACGCTTTATTGGAATGATATGGAGGGTTATCTAGAA CAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCA TCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMMWFRFLLVALLWSLGSATIMENGQPRADPYPGQCSTIDLDSSW RSYDADAPEISYKGRAPGLKLQFSGKKLALSFGEHTSEGTLVAYRIGTLD WLFTNVTADATYQFVGEGTHYEELPGDGDHIFEMRVQIAGVSVDVDDHLT KPPTFKKKVEIIGGSASLTGGQYATYETLSAWSFVYAQGLGNVEFGITAY PGACLVDMQCYGGGVHGMIWYWHRASDPGSRARNMYGGEPEEWDVTAEQP ADLVIIQMGGNDHRHPNEIPGENFYQGYVDLIENIHQHWPDASIVIMTQW GIWNQEGSEYTQSVIYEEEIQRVYEHFKDRKWRFVYLFHTDGILGHNDIN PKNHPTDVGHIKIASHLLQWTNLMLGWELNPRGEMQHGTLYWNDMEGYLE QKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGATGTGGTTTCGCTTT CT (Pml I) TACAGTCTAGATAACCCTCCATATCATTCCA (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on naphthyl acetate, PNP-acetate
References: not yet available