Enzyme Function:pectin lyase
Accession number: AN2569.2
GenBank ID: DQ490480
FGSC number: 10080
Average molecular weight
(clone, calculated): 		42.649 kDa
AA length (clone): 407 aa
DNA length (clone): 1224 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAGGCTTCACGCACCCATTCTGTCTCTTTT GGCTGCCGCTGCCAGCACCAGCGCCGCCGGGGTGACAGGATCAGCCGAGG GCTTCGCAAAGGGCGTGACTGGAGGTGGGAGTGCGACGCCGGTTTATCCA AGCACTACTGCAGAGCTAGTCTCGTACCTGGGAGACAGTTCGGCCCGCGT TATTGTCTTGACCAAGACGTTCGACTTCACGGGAACCGAGGGTACTACGA CAGAGACTGGCTGCGCCCCCTGGGGCACTGCGTCGGCCTGCCAAGTGGCC ATCAACAAGAACGACTGGTGTACAAACTACCAGCCTAACGCTCCGTCGGT TTCTGTAACCTATGACAACGCCGGCGTGTTGGGTATCACTGTCAAATCCA ACAAAAGCCTCGTGGGTGAAGGATCCAGTGGTGTGATCAAAGGCAAGGGT CTTCGAATTGTCAGCGGCGCAAGCAATGTCATCATCCAAAACATCGCTAT CACGGACCTGAACCCTAAGTATGTCTGGGGCGGTGATGCAATCACCCTCG ACAATGCGGATATGGTTTGGATCGACCATGTCACGACTGCACGCATTGGG CGACAACATCTTGTCCTGGGGACATCTGCCTCTAACAGAGTAACCGTTTC CAATTCGTACTTCAACGGCGTGACCTCCTACTCCGCCACCTGCGATGGCT ACCACTACTGGGGCATTTACTTGACTGGTTCGAACGACATGGTCACTCTG AAGGGGAACTATATCTACCACATGAGCGGTCGCAGCCCTAAAGTCGGCGG GAACACTCTTCTGCATGCGGTCAACAACTACTGGTATGACTCGTCCGGCC ATGCCTTTGAAATTGACTCTGGTGGCTATGTGCTTGCAGAAGGCAACGTC TTCCAGAATATCCCTACTGTTATTGAAGGGACCGTCGGCGGCCAGCTGTT CACCTCCCCAGATTCCAGCACTAATGCAATCTGCTCGACATACCTGGGCC ACACCTGCCAGGTCAATGGCTTCGGGAGCTCTGGAACGTTCAAGCAGGCA GACACGGCGTTCTTAGTGAATTTCCAGGGCAAGAACATTGCGTCTGCATC TGCGTATACCGTTGCGCAGAGTAGTGTTCCGTCCAATGCTGGGCAGGGGA AACTTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTC GACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMRLHAPILSLLAAAASTSAAGVTGSAEGFAKGVTGGGSATPVYP STTAELVSYLGDSSARVIVLTKTFDFTGTEGTTTETGCAPWGTASACQVA INKNDWCTNYQPNAPSVSVTYDNAGVLGITVKSNKSLVGEGSSGVIKGKG LRIVSGASNVIIQNIAITDLNPKYVWGGDAITLDNADMVWIDHVTTARIG RQHLVLGTSASNRVTVSNSYFNGVTSYSATCDGYHYWGIYLTGSNDMVTL KGNYIYHMSGRSPKVGGNTLLHAVNNYWYDSSGHAFEIDSGGYVLAEGNV FQNIPTVIEGTVGGQLFTSPDSSTNAICSTYLGHTCQVNGFGSSGTFKQA DTAFLVNFQGKNIASASAYTVAQSSVPSNAGQGKLLEQKLISEEDLNSAV DHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAGGCTTCACGCACCC (Pml I) TACAGTCTAGAAGTTTCCCCTGCCCAGC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on citrus pectin
References: not yet available