Enzyme Function:rhamnogalacturonan acetylesterase
Accession number: AN2528.2
GenBank ID: DQ490479
FGSC number: 10079
Average molecular weight
(clone, calculated): 		29.203 kDa
AA length (clone): 273 aa
DNA length (clone): 822 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGAAATCCATCGCTCTTACCTCTCTTTCTCT CCTCCCGTCCGCGCTGGCGCAGACAATTTACTTGGCTGGTGACTCCACCA TGGCGTCCAGTACTCCTGGATGGGGTGACTACATTGCCGACAGCGTCTCG GTTGAAATCTCTAACCAGGCCATTGGTGGTCGTAGCGCGCGCTCCTACAC CCGCGAGGGCCGCTTCCAGGCCATCGCCGACGTCCTTCAAGCAGGCGACT ATGTTGTTATCGAGTTTGGACACAACGACGGCGGCTCTCTCTCCAACGAT AACGGCCGTACTGACTGCCCTGGCGATGGCGATGAGACCTGTGAGACCGT GTACAACGGGGTGGCCGAAACTGTCCTCACCTTCCCAGCGTACATCGAAA ATGCGGCGCTGCTGTTTCTGGAGAAGGGTGCCAACGTCCTGATCTCCAGC CAGACGCCCAACAACCCATGGGAGAGCGGAACATTCTCCTACACGCCGAA CCGATTTGTCGGGTATGCCGAGTTGGCGGCTCAAAGGGCAGGAGTAGACT ATGTGGATCATGGAGCTTATACAGCGAGCATCTTTGAGGCGCTCGGCGCA GACACGGTCAACTCGTTCTACCCGAATGACCATACGCATACTAACGCCGA GGGCTCCAGTGTTGTTGCTGATGCTTTCCTCAAGGCCGTTGTGTGCAGCG GGGTTGCGTTGAACGATGTCTTGACAAGAACTGATTTCGACGGTGAATGC TTTCTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGA CCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMKSIALTSLSLLPSALAQTIYLAGDSTMASSTPGWGDYIADSVS VEISNQAIGGRSARSYTREGRFQAIADVLQAGDYVVIEFGHNDGGSLSND NGRTDCPGDGDETCETVYNGVAETVLTFPAYIENAALLFLEKGANVLISS QTPNNPWESGTFSYTPNRFVGYAELAAQRAGVDYVDHGAYTASIFEALGA DTVNSFYPNDHTHTNAEGSSVVADAFLKAVVCSGVALNDVLTRTDFDGEC FLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGAAATCCATCGCTCTTAC (Pml I) TACAGTCTAGAAAGCATTCACCGTCGAAA (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-acetate, naphthyl acetate
References: not yet available