Enzyme Function:mixed-linked glucanase
Accession number: AN2385.2
GenBank ID: XM_654897
FGSC number: 10078
Average molecular weight
(clone, calculated): 		68.396 kDa
AA length (clone): 654 aa
DNA length (clone): 1965 bp
DNA source: genomic DNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGTCTTCTTCCCTCATGCGAAGGGTGGGTTC CCTCGCCGCCTCCGCGATCATCTTCCCCGGCATCGCCCATGCCGCCAGCA ACTACAAACTCAAGGAGTCCTGGGAAGGCGAGAAGATCCTTAATCACTTC CACTTCTTTGACAACGCCGACCCCACCAACGGTTTCGTCACCTACGTCAA TCAGAGCTACGCAGAGTCCGCCGGCCTCGTCAAGACCACCGACAGCGGGA GCCTCTATTTAGGTGTTGACTATGAGAACGTTCTCACCGTAGACGGCCCG GGCCGTGAGTCTGTCCGCATCGAGAGCAACGAGTATTACGACCAGGGGTT GTACGTCGTCGATATCCAACACATGCCCGGCAGCATCTGCGGCACATGGC CAGCCTTCTGGACCGTTGGCCCCGACTGGCCGACGGACGGTGAGATTGAT ATTATCGAGGGAGTGAACAAGCACGATGCGAACAAGATCGTGCTGCACAC TAGCGATACTTGTGACGTTGGTGGTGGCTACAAGATGACCGGCGACATGA CCTCAAGCGAATGCGGCGAAGCTTCAGGAACTATCGGATGCGTTGTCCAA GGCAAGCAGGGCTCCTCCGGCGACCCTTTCAACGAGCAAGGTGGTGGAGT CTATGCCATGGAATGGCAGGAAAAGTACCTCAAGATCTGGTACTTTCCCC GGTCTTCGATCCCCGAGTCCCTCACTGCGGGCACCCCTGACGTTTCGTCT TTTGGCACTCCTATGGCTCACCTCCAGGGCTCCTGCAACTTCAAGGAACG TTTCACCCATCAGAAGCTGATCCTCGACACAACATTTTGCGGTGACTGGG CGGGCGGTGTTTTTGGGGACAGCGGATGCCCCGTCAGCGACCCCAGCGAC CCAATGCTGAGTTGCAAGAACTATGTTGCCGAGAACCCGGCTGTATACAA GAACGCTTACTGGGAGCTGAACTCTATTAAGATCTACCAGCTTGGTGGGA CTGCTGAGGTGGAGGGCACCCAGTCCGCTGCCGCCGAGTCTACTGCCGCC GAGGCCACTGCTGCCGAAACGACTGCTGCTGCGACGCAGACGGCTAATGG GGGCTCAATTGAGGAGATTACTACCAGCACCCACAGCGTTACCCGGACAA AGACCGTGAGCGCCACGCACTCAACTGAGACTGCCGCTGTAACTGAGACT GCTGCTGCAACTACGGCTGCCGCCAGTGTTGCTTCGGAAGTTGACGCAAC CAACACGCAACCCGTCTCCAAGACTAAGAGCACCAGCTACGTCACCTCCA CAACCACACTATGTCCCGTCGAGAGCTCCCAGGCGGCCGCCACGGAATCC GTCTCCAGAACAAAGACAACCAGCTACGTGACGATCACGACGACGCTCTG CCCAGTCGAGAGCCTGCAGACTGCCAATGCCGTTCCCAGCGCAAAGGCCT CAACAGACGCAGCTGCAGCCACAACCCCGGCGGCTGAACCTCACCCATCC AACGCGGAGACCCCCGCAGACTCGAAGAGCTCTGCAGACGCCGTGACCGC CCAAGCCACGAAGACAACCATCGCCGTGAACACCCCTAACCCGGCGACCG ACTCAGCCTCTTCGGTTCCTCCAGATAGCATCGTCTACACAGCGCCAGAA GTGACCAGCAGCTCGTCCGTTCCACTGTTCACTATCGTCTCGTCCTCCTC GCAGTTCGTGACTGTCCCTACTGCGGCCCCATCGTCCTTCGAGCCTACGG ACGCTGTGCGCGACGGAGCCGACAGCTACAGCACAGCTGCATCCCCGACG ACGCCGTCCAACCCCGTCTTCACGGGTGTAGGTAGCAAGGTTTCTATCAG CGCGTCTGTAGCCATTGCTGCCTTTGTGATGCTGCTGCTCGTCAATCTAG AACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCAT CATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMSSSLMRRVGSLAASAIIFPGIAHAASNYKLKESWEGEKILNHF HFFDNADPTNGFVTYVNQSYAESAGLVKTTDSGSLYLGVDYENVLTVDGP GRESVRIESNEYYDQGLYVVDIQHMPGSICGTWPAFWTVGPDWPTDGEID IIEGVNKHDANKIVLHTSDTCDVGGGYKMTGDMTSSECGEASGTIGCVVQ GKQGSSGDPFNEQGGGVYAMEWQEKYLKIWYFPRSSIPESLTAGTPDVSS FGTPMAHLQGSCNFKERFTHQKLILDTTFCGDWAGGVFGDSGCPVSDPSD PMLSCKNYVAENPAVYKNAYWELNSIKIYQLGGTAEVEGTQSAAAESTAA EATAAETTAAATQTANGGSIEEITTSTHSVTRTKTVSATHSTETAAVTET AAATTAAASVASEVDATNTQPVSKTKSTSYVTSTTTLCPVESSQAAATES VSRTKTTSYVTITTTLCPVESLQTANAVPSAKASTDAAAATTPAAEPHPS NAETPADSKSSADAVTAQATKTTIAVNTPNPATDSASSVPPDSIVYTAPE VTSSSSVPLFTIVSSSSQFVTVPTAAPSSFEPTDAVRDGADSYSTAASPT TPSNPVFTGVGSKVSISASVAIAAFVMLLLVNLEQKLISEEDLNSAVDHH HHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGTCTTCTTCCCTCATGCG (Pml I) TACAGTCTAGATTGACGAGCAGCAGCATC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on laminarin, lichenan, soluble CMC, not active on pustulan
References: not yet available