Enzyme Function:beta-glucosidase
Accession number: AN2227.2
GenBank ID: DQ490477
FGSC number: 10075
Average molecular weight
(clone, calculated): 		95.240 kDa
AA length (clone): 866 aa
DNA length (clone): 2601 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGCCTCAATTGGATGTGGACAAGACGATTGA AGAACTTCGTCTCGGAGAGAAGATCGATCTTGTGTCAGGGATCGACTTCT GGCATACTGCATCTGTCCCCAGGCTTAACATTCCCAGCCTGCGCATGTCC GATGGCCCCAATGGAGTCCGCGGAACGCGGTTTTTTAACGGTGTGCCAGC AGCATGTTTCCCCTGCGCTACTGCCCTGGGAGCAACCTGGGACACTGAAT TACTGCACAAGGTAGGGCATTTGATGGGGGAAGAGGCCATTGCCAAGGGC GCGCACGTCATATTGGGTCCCACAATCAATACGCAACGATCGCCGCTGGG CGGCCGAGGGTTCGAGTCTTTCGCCGAAGATGGTGTTCTTGCCGGCCACT TGGCTGGCTACTGTTCCAAGGGAATCCAGGAAAAGGGCGTTGCCGCGTGC CTGAAGCACTTTGTGTGCAATGACCAGGAGCATGAGCGTTTGGCGGTCGA TTCGATTGTTACCGACCGGGCTACACGCGAGATATACCTCTTGCCGTTCC AGATTGCGATGAGGATTTGCAAAACGGCCACAGTTATGACGGCTTATAAC AAGATTAACGGTACCCATGTGAGTGAGAATAAGAAGTACATCACAGACAT TTTGCGCAAGGAGTGGGGATGGGACGGACTAGTTATGAGTGACTGTACGT CAGAATCAATAATTGCCGGTCTCGACATAGAGATGCCAGGTAAGACACGC TGGCGGGGAGACGCCCTGGCACACGCCGTATCATCCAACAAAGTCCACGA GTTTGTCCTGGATGAACGTGTACGCAACGTTCTGAACCTTGTAAATTACG TTGAGCCACTGGGTATCCCCGAAAACGCAGAGGAAAAGGTTCTTAACCGC CCCGAAGACCAAGCCCTACTCCGACGGGCTGCGGCCGAGTCTATCGTGCT CTTGAAGAACGAGGATAACATCCTACCTTTCAACAAGGAGAAGTCCATCG CCGTGATCGGACCAAACGCCAAGATCGCCGCTTACTGTGGAGGTGGCTCT GCATCCTTGGATGCGTACTACACAATCACACCCTTCGAAGGCGTATCAGC ACAGAGCAAGGGTGAGGTTCACTTCGCCCAAGGTTCATACTCATACAAGG ACCTTCCGTTGATCGGCCACCTCCTGAAGACCGACGACGGCAAAACGGGC TTCAAGTTCCGCGTTTACGACGAGCCCGCATCCAGTAGCAACCGCGAACT CCTCCACGAGCTACACCTTGTCTCATCCCAGGGCTTCCTCATGGACTATC GCCACCCTAAGATCAAGTCTTACCTTTACTACGTCGACATGGAGGGCTAT TTCACTCCCGAAGAGTCCGGTGTCTACGACTTCGGCGTTGTTGTCGTCGG AACGGGGAAACTCCTCGTGGATGATGAAGTCGTCGTTGATAACACCAAGA ACCAGCGTCTCGGCTCTGCCTTCTTTGGAAACGGCACCGTTGAAGAAAAG GGCTCCAAGGAACTCATGGCTGGCCAGAAATACAAAATTACCTTCCAGTT CGGGACAGCACCCACCTCGGACATCGACACCCGCGGGGTGGTCATCTTCG GGCCTGGAGGCTTCCGCTTCGGCGCTGCCCGGCGCCAGACGCAGGAAGAG CTCATTTCCAAAGCTGTCGAAGTCGCCTCAAAAGCAGACCAGGTTGTCGT CTTCGCTGGTCTAACAAGCGAATGGGAAACAGAAGGGTACGATCGTCCGG ACATGGATCTTCCCCCGGGTTCAGACGAACTGATTTCCAAAATCCTGGAG GTGAAACCCAACGCCGCCATCGTCATCCAGAGCGGTACGCCCGTGACAAT GCCCTGGGCGCCCAAAGCCAAGGCTCTTCTGCAGGCTTGGTTCGGGGGCA ATGAGTGTGGTAACGGCATCGCAGATGTGCTTTATGGAAACGTCAACCCC TCTGGAAAGCTGCCTTTGACCTTCCCCGTCCGTTTGCAAGACAACCCTAG TTACCTGAACTTCCGCTCAGAGCGAGGCCGCGTCCTCTACGGCGAAGACA TCTATGTTGGGTACCGGTACTACGAGAAAGCCCAACTCCCGCCGTTATTC CCCTTCGGCCATGGCCTGTCCTACACGACTTTCACACGCGAGAAACTGGA GTTGAACACTTCTCCTGAAAAAGACAAACTGCAAGACGGAGAACCCATAA CCGCCAGGGTGACCGTCACAAACACGGGCAAAGTCGTCGGCGCCGAAACA GTGCAGCTGTGGGTTGTGCCGCCCCCGACAGAGGTGAACAGACCTGTCCG CGAACTAAAGGGTTTTGCGAAGGTGCACCTGGAACCTGGAGAGTCAAAAG ATGTCGAGATTGTGGTTGAAAAGAAGCTGGCGACGAGCTGGTGGGATGAG AAACGGGAAGCCTGGGCATCAGAGAAAGGAGTTTATTGGGTGCAAGTTAC GGGGACGGGAGAGGGGGTTCTCACTGCAGAGTTTGAGGTTAAGAAGACGA GGTTCTGGACCGGATTTCTAGAACAAAAACTCATCTCAGAAGAGGATCTG AATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMPQLDVDKTIEELRLGEKIDLVSGIDFWHTASVPRLNIPSLRMS DGPNGVRGTRFFNGVPAACFPCATALGATWDTELLHKVGHLMGEEAIAKG AHVILGPTINTQRSPLGGRGFESFAEDGVLAGHLAGYCSKGIQEKGVAAC LKHFVCNDQEHERLAVDSIVTDRATREIYLLPFQIAMRICKTATVMTAYN KINGTHVSENKKYITDILRKEWGWDGLVMSDCTSESIIAGLDIEMPGKTR WRGDALAHAVSSNKVHEFVLDERVRNVLNLVNYVEPLGIPENAEEKVLNR PEDQALLRRAAAESIVLLKNEDNILPFNKEKSIAVIGPNAKIAAYCGGGS ASLDAYYTITPFEGVSAQSKGEVHFAQGSYSYKDLPLIGHLLKTDDGKTG FKFRVYDEPASSSNRELLHELHLVSSQGFLMDYRHPKIKSYLYYVDMEGY FTPEESGVYDFGVVVVGTGKLLVDDEVVVDNTKNQRLGSAFFGNGTVEEK GSKELMAGQKYKITFQFGTAPTSDIDTRGVVIFGPGGFRFGAARRQTQEE LISKAVEVASKADQVVVFAGLTSEWETEGYDRPDMDLPPGSDELISKILE VKPNAAIVIQSGTPVTMPWAPKAKALLQAWFGGNECGNGIADVLYGNVNP SGKLPLTFPVRLQDNPSYLNFRSERGRVLYGEDIYVGYRYYEKAQLPPLF PFGHGLSYTTFTREKLELNTSPEKDKLQDGEPITARVTVTNTGKVVGAET VQLWVVPPPTEVNRPVRELKGFAKVHLEPGESKDVEIVVEKKLATSWWDE KREAWASEKGVYWVQVTGTGEGVLTAEFEVKKTRFWTGFLEQKLISEEDL NSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGCCTCAATTGGATGTGG (Pml I) TACAGTCTAGAAATCCGGTCCAGAACCTC (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description:
Warning: include(/usr/local/apache/htdocs/research/csomerville/enzymes/description/2227_desc.txt) [function.include]: failed to open stream: No such file or directory in /opt/apache/htdocs/research/csomerville/enzymes/enzyme-template.php on line 345

Warning: include() [function.include]: Failed opening '/usr/local/apache/htdocs/research/csomerville/enzymes/description/2227_desc.txt' for inclusion (include_path='.:/usr/local/lib/php') in /opt/apache/htdocs/research/csomerville/enzymes/enzyme-template.php on line 345
References: not yet available