| Enzyme Function: | oligoxyloglucan reducing end-specific xyloglucanase |
| Accession number: | AN1542.2 |
| GenBank ID: | XM_654054 |
| FGSC number: | 9925 |
| Average molecular weight (clone, calculated): | 90.396 kDa |
| AA length (clone): | 838 aa |
| DNA length (clone): | 2517 bp |
| DNA source: | genomic DNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGAGATGAGGGCGAAGAACGGACCCGGCAGCTGGCT GGCCCTTACAGCCATTGCCACATCTTTGAACACCCTCGCTCTCGCCGCAG CAAAGACAATTAACCACTCCTACGAATTCAACCCCGTCGTCGTCTCGGGT GGCGGCTATATCACAGGCATAATCGCCCACCCCACGGAAAAGAACCTGCT CTACGCGCGCACAGACATCGGGGGAACATATCGGTGGAATGCGGACGAGG ACAAATGGATCCCGTTAAACGATTTCATCAGTGGCGCGGACGAGAACTTA CTCGGGACGGAGAGTGTTGCCCTGGACCCCAATGATCCGGATCGGCTCTA CCTGGCGCAGGGACGGTATCTAAATTCAGAGAATTCCGCCTTCTTTGTCT CGCAAGATAGAGGGGCGACATTCGATGTTTACCCGGCGCCGTTTAAGATG GGTGCCAATGAGCTGGGGCGGAATAATGGGGAGAGACTAGCTGTGAACCC GTTTAAGACGGACGAGCTCTGGATGGGGACTAGGGATGCGGGCTTGATGG TGAGTGAGGATGGGGCGCAAACTTGGAGAAATGTGAGCGGGTTCCCTCAG GCGAATGCGAACGGAATTGGGATCTACTGGGTGATATTTGACCCCAGGAG CGAGGGGACGGTCTATGTTGGCGTCGGCGTGCCTGGGGGGATCTATGTTA CGAGGGACTCTGGAGAGAGTTGGGAGGCTGTACCTGGACAGCCAGTAGAG TGGGATGAGGATATCCTGGTCTTTCCAGCTGAATCGCAGCCGCAGAGCAC TGGACCACAGCCGATGAAAGGTGTTTTAGCAGAAAATGGCGCATTGTATG TGACCTATGCCGATGCACCTGGTCCGTATGGTGTGACATATGGCGGAGTG TATGTCTACAACACGACTTCATCTGCGTGGACCAATATCACGCCGAAAAC TAACAACTCGTTTCCGGCGCCATTCGACAACCAGACTTTCCCGGCGGGAG GATTCTGTGGGATCAGTGTAGATTCAAAAAATCCGGAAAGGCTTGTGGTG GTATCCCTCGATCGCGATCCCGGTCCGGCTCTGGACAGTATGTACCTCTC GCACGACGGTGGGAAGTCCTGGAAAGATGTGTCTCAGCTTTCGACACCAT CTGGCTCAGGCGGGTACTGGGGCCATCCGATTGAGGAAGCTGCGTTCAAA GATGGTACCGCTGTTCCCTGGCTCAGCTTCAACTGGGGCCCTCAATGGGG CGGTTACGGTGCCCCTTCTCCCGTTCGTGGGCTGACAAAGTTTGGATGGT GGATGACGGCAGTTGTTATCGACCCGAGTGACTCTGACCATGTTCTTTAT GGCACCGGAGCGACTATCTGGGCAACTGACAACTTGTCCAAGGTCGACAA GAACCAGTCCCCTGGCTGGTACATTCAAGCTCAAGGCATTGAAGAATCGG TTGCTTTAGCCTTAGCAAGCCCGAACGGCGGCGATTCGCACCTTCTCACC GGTCTGGGTGATATCAACGGATACCGGTATGGAGATCTCGACGTTCCGCA GCCAATGTTCGACTTACCGGTGTTGTCAAACTTGAATGCTCTCGACTGGG CTGGTCAGAAGCCGGAAATTATCATCCGTGCCGGCCCTTGCGGACACAAC TATACTGACGGCTGCGGACTCGCGGCGTATTCCGCGGACGGTGGCAGTTC GTGGACCAAGTTCGCAACCTGCATTCCTGGCATAAACACAAGCTCCTCGA ATCCCGGGGTCATTGCGATCGATGCTTCAGGCAAGGATATCGTGTGGTCG TCGGCAATGACAGCGTATTGGCCGACTCTGCAGGCTATCACCCCTCGTAC GAACCAATCCGGTCCATACGTCACCACTGACTTAGGACAGACTTGGGTGT CTCCAACTGGCTTGAATGTGCAGACTCCCAACATTAGCGCTGACAGAGTC CAGCCACGAACATTTTACTCCTTCACTGATGGAACATGGTATCTGAGCAG GGATGGCGGCCTCTCCTACCGCGCCTACAAAGCTAAAGAGGTCGGGCTGC CAGCATACTCGGGAGCGCTGCCAATTGCGAATTTCAACAGAGCAGGCGAG ATATGGCTGGGCCTGGGGGATCATGGAATCTACCATACCCGCAATTTTGG CAAGAAATGGACAAAGATCACTGGACGCGGAGTGACAGCAAGACAGCTAA CAATTGGTGCCGGAGCGCGTCGCTCATCGGAACCAACACTCTTCATTGTT GGAAAAGCCGCGAGCCATGGTGCTCTCAGTAAAGACGGAGTATACCGATC AGACGATAATGGTAAGACTTGGGTGCGAGTCAATGACGAGAAGCATCAGT ACGGTGGAATTGCAATGATACAAGGCGATCCGCGCGTGTACGGACGTGTC TATCTGGGTACCGGCGGTCGCGGTATTATCTATGCCGATATCAAGGATCT AGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATC ATCATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSREMRAKNGPGSWLALTAIATSLNTLALAAAKTINHSYEFNPVVVSG GGYITGIIAHPTEKNLLYARTDIGGTYRWNADEDKWIPLNDFISGADENL LGTESVALDPNDPDRLYLAQGRYLNSENSAFFVSQDRGATFDVYPAPFKM GANELGRNNGERLAVNPFKTDELWMGTRDAGLMVSEDGAQTWRNVSGFPQ ANANGIGIYWVIFDPRSEGTVYVGVGVPGGIYVTRDSGESWEAVPGQPVE WDEDILVFPAESQPQSTGPQPMKGVLAENGALYVTYADAPGPYGVTYGGV YVYNTTSSAWTNITPKTNNSFPAPFDNQTFPAGGFCGISVDSKNPERLVV VSLDRDPGPALDSMYLSHDGGKSWKDVSQLSTPSGSGGYWGHPIEEAAFK DGTAVPWLSFNWGPQWGGYGAPSPVRGLTKFGWWMTAVVIDPSDSDHVLY GTGATIWATDNLSKVDKNQSPGWYIQAQGIEESVALALASPNGGDSHLLT GLGDINGYRYGDLDVPQPMFDLPVLSNLNALDWAGQKPEIIIRAGPCGHN YTDGCGLAAYSADGGSSWTKFATCIPGINTSSSNPGVIAIDASGKDIVWS SAMTAYWPTLQAITPRTNQSGPYVTTDLGQTWVSPTGLNVQTPNISADRV QPRTFYSFTDGTWYLSRDGGLSYRAYKAKEVGLPAYSGALPIANFNRAGE IWLGLGDHGIYHTRNFGKKWTKITGRGVTARQLTIGAGARRSSEPTLFIV GKAASHGALSKDGVYRSDDNGKTWVRVNDEKHQYGGIAMIQGDPRVYGRV YLGTGGRGIIYADIKDLEQKLISEEDLNSAVDHHHHHH |
| Primers Used (Restriction sites): | CACGACACGTGAGATGAGGGCGAAGAAC (Pml I) TTGTGCTCTAGATCCTTGATATCGGCATAG (Xba I) |
| pH optimum: | 3.0 |
| Specific activity (Substrate): | tamarind XG oligos |
| T optimum: | 42 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | MM |
| Description: | less active on tamarind XG, but highly active on XG oligomers, and removes the first two glycosyl segments from the reducing end providing the second xylose from the reducing end is not further substituted |
| References: | Bauer, S., Vasu, P. Mort, A.J., Somerville, C.R. (2005) Carbohydr. Res. 340, 2590-2597 |
oligoxyloglucan reducing end-specific xyloglucanase