| Enzyme Function: | endo-beta-1,4-glucanase |
| Accession number: | AN1285.2 |
| GenBank ID: | DQ490472 |
| FGSC number: | 10068 |
| Average molecular weight (clone, calculated): | 39.119 kDa |
| AA length (clone): | 354 aa |
| DNA length (clone): | 1065 bp |
| DNA source: | pectin cDNA |
| DNA Sequence (clone): | TCGATGAATTCACGTGATATGAGGTCTCTCGTCCTTCTGTCGTCCGTCCT GGCCCTGGTGGCACCCTCCAAAGGCGCCTTCACATGGCTTGGTACCAACG AAGCCGGTGCCGAATTCGGCGAGGGCTCCTACCCCGGCGAACTGGGCACG GAATACATTTGGCCTGACCTGGGTACGATTGGTACGCTGAGGAACGAGGG GATGAACATATTCCGCGTTGCGTTCTCCATGGAGCGCCTGGTGCCTGATT CGTTGGCTGGACCGGTAGCGGACGAGTATTTTCAGGACTTGGTCGAGACG GTCAACGGCATTACGGCCCTGGGTGCGTATGCAGTCCTTGACCCGCATAA TTATGGACGGTACTACGGCAACATTATCACTTCAACCGATGACTTTGCAG CCTTCTGGACCATCCTTGCCACCGAGTTCGCTTCTAATGAGCTTGTCATC TTCGATACCAATAATGAATACCACACCATGGACCAGTCCCTTGTCCTGAA CCTCAATCAGGCGGCCATAGACGCTATTCGCGCCTCCGGTGCCACCAGCC AATATATCTTCGCGGAAGGAAACTCTTGGACTGGAGCCTGGACCTGGGTG GATGTCAACGACAACATGAAAGCGCTCACCGATCCCCAAGATAAGCTCAT CTACGAGATGCACCAGTATCTTGACTCCGACGGCTCTGGGACGAACACGG CCTGCGTCAGTTCTACTATCGGCAGCGAACGCGTGACAGCCGCTACAAAC TGGCTGCGAGAGAACGGGAAACTGGGGGTGCTCGGTGAGTTTGCAGGCGC AAACAACCAGGTCTGCAAGGACGCAGTCGCAGATCTGCTGGAGTATCTTG AGGAAAACAGTGATGTATGGCTTGGGGCCCTTTGGTGGGCTGCTGGGCCT TGGTGGGGTGATTATATGTTCAACATGGAGCCTACTTCGGGTATTGCATA CCAGGAGTACTCGGAAATCTTGCAGCCGTATTTCGTGGGAAGTCATCTAG AACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCAT CATCATCATCATTGA |
| Amino Acid Sequence (clone): | SMNSRDMRSLVLLSSVLALVAPSKGAFTWLGTNEAGAEFGEGSYPGELGT EYIWPDLGTIGTLRNEGMNIFRVAFSMERLVPDSLAGPVADEYFQDLVET VNGITALGAYAVLDPHNYGRYYGNIITSTDDFAAFWTILATEFASNELVI FDTNNEYHTMDQSLVLNLNQAAIDAIRASGATSQYIFAEGNSWTGAWTWV DVNDNMKALTDPQDKLIYEMHQYLDSDGSGTNTACVSSTIGSERVTAATN WLRENGKLGVLGEFAGANNQVCKDAVADLLEYLEENSDVWLGALWWAAGP WWGDYMFNMEPTSGIAYQEYSEILQPYFVGSHLEQKLISEEDLNSAVDHH HHHH |
| Primers Used (Restriction sites): | GAAAGCACGTGATATGAGGTCTCTCGTCCTTCT (Pml I) TACAGTCTAGATGACTTCCCACGAAATACG (Xba I) |
| pH optimum: | 4.0 |
| Specific activity (Substrate): | CMC |
| T optimum: | 57 |
| ph Range Link: | Link |
| Temperature Link: | Link |
| Optimal Expression Medium: | BMMY |
| Description: | active on soluble CMC, active on cellooligos Glc4/Glc5/Glc6 producing mainly Glc2 and Glc3, active on barley beta-glucan and lichenan |
| References: | not yet available |
endo-beta-1,4-glucanase