Enzyme Function:alpha-L-arabinofuranosidase
Accession number: AN1277.2
GenBank ID: DQ490471
FGSC number: 10067
Average molecular weight
(clone, calculated): 		59.853 kDa
AA length (clone): 532 aa
DNA length (clone): 1599 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGACGACCTTCACGAAGCTCAGTGAGCAGGA AGCTCCGGGAATTTCCATACACCCAGACCGCCGCATCTCAAAAATCAATC CTAATATCTATGCCGGGTTTGCAGAGCACATGGGTCGATGCATCTACGGG GGCATCTACGATCCCGGTAACCCATTATCCGATGAGAATGGATTTCGAAA GGACGTCCTCGAAGCCCTTAAGGAGCTCAAGGTCCCTGTCATTCGTTACC CTGGTGGCAACTTCACCGCAACATACCATTGGATCGATGGAGTTGGTCCG AGAGACCAACGTCCCGCGAGGCCGGAACTTGCGTGGCTGGGCACAGAGAC CAACCAGTTTGGAACTGATGAGTTTTTGAAGTGGTGTGAGGTGCTGGGAA CCGAGCCCTATCTTTGCCTGAACATGGGCACGGGTACTCTTGATGAGGCT TTGGCTTGGGTCGATTATTGCAACGGTACCCGGGACACCTACTACGCCAA TCTCCGACGCAAGAACGGACGAGAAGAACCGTACAATGTCAAATATTGGG CCCTCGGTAACGAAGTATGGGGACCATGGCAGGTTGAGCAGTCTACCAAG GAGGAATATGCTCACAAGGCCTACCAATGGGCTAAAGCCCTGAAACTCCT TGATCCTTCTATCGAGTTGATTCTGTGCGGAAAAGAAGGCCCAACATCGT GGGACTATTACACCCTAAAGCAAACCATGCTCCCCGTGCACTCGCCCCTA TCAACAAGCGCAGTCCCCCTCATCGATATGCACAGTATCCACCTCTACAC AGCTCACTCCTCACACCTTCCTAACGTCACCGCGCCTCTAGCCGCTGAGC GCGCCATCGAGATCACCTCCTCTCTCATCGACCTCGCCCGGGTCGAGAAC GGGGTCCCGCCCGAGCAACGCCGCCCGACTATCTGCTTTGATGAGTGGAA CGTCTGGGACCCCATCCGTGCCGAGGGCAGTAAAGGCGCAGAGGAATCCT ACAACCTCTCCGATGCTTTAGCTGTGGGTGTCTGGCTTAACGTGTTTGTG CGTAAGAGTAAGGATGTTGGTATGGCGTGCATTGCGCAGAGCGTCAATGT AATTTCGCCGCTTATGACCACGAAAGATGGGATCGTGAAGCAGACGACGT GGTGGCCGCTCTATCTGTTCTCGAATTACATGCGTGGCTGGACTATCAGT GCACACATTTCTGTTTCTGCATACGAGGGCGAAACACACCCGAAATGGGT AAGGGGTGTCAAGGATACGCCTTGGTTGGACGTTAGTGCGACTCTGGGTG AGGATGGATATGTCAATGTAGTTGTGATCAACATCCATGAAGAGAAGGGG ATCGAGGCGAAGCTTGATGGTCCATCTGGTGAGGTTACTGTGTTCACTGT TACCGGTGACAACCCAGCCGTGACGAACATGAAGGGCAAGGAAGAAGTTG GTCTGGTTGAGACCAAGTGGGATGCCCAGGGCCCATACGTGTTCCCGAAG CATTCCCTTACCCTGCTGAGATGGAAGGCTCTAGAACAAAAACTCATCTC AGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRDMTTFTKLSEQEAPGISIHPDRRISKINPNIYAGFAEHMGRCIYG GIYDPGNPLSDENGFRKDVLEALKELKVPVIRYPGGNFTATYHWIDGVGP RDQRPARPELAWLGTETNQFGTDEFLKWCEVLGTEPYLCLNMGTGTLDEA LAWVDYCNGTRDTYYANLRRKNGREEPYNVKYWALGNEVWGPWQVEQSTK EEYAHKAYQWAKALKLLDPSIELILCGKEGPTSWDYYTLKQTMLPVHSPL STSAVPLIDMHSIHLYTAHSSHLPNVTAPLAAERAIEITSSLIDLARVEN GVPPEQRRPTICFDEWNVWDPIRAEGSKGAEESYNLSDALAVGVWLNVFV RKSKDVGMACIAQSVNVISPLMTTKDGIVKQTTWWPLYLFSNYMRGWTIS AHISVSAYEGETHPKWVRGVKDTPWLDVSATLGEDGYVNVVVINIHEEKG IEAKLDGPSGEVTVFTVTGDNPAVTNMKGKEEVGLVETKWDAQGPYVFPK HSLTLLRWKALEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAGCACGTGATATGACGACCTTCACGAAGC (Pml I) TACAGTCTAGAGCCTTCCATCTCAGCAGG (Xba I)
pH optimum: n.d.
Specific activity (Substrate): n.d.
T optimum: n.d.
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: not active on PNP-alpha-arabinofuranoside and sugar beet arabinan, but active on Ara7
References: not yet available