Enzyme Function:alpha-glucosidase
Accession number: AN0941.2
GenBank ID: DQ490470
FGSC number: 10066
Average molecular weight
(clone, calculated):
101.342 kDa
AA length (clone): 902 aa
DNA length (clone): 2709 bp
DNA source: pectin cDNA
DNA Sequence (clone): TCGATGAATTCACGTGATATGTTGGGATTTCTACTTTTCCTGCCGCTAGT CGGCGCCGCCGTCATTGGGCCCAGGGCGAACAGCCAAATTTGTCCTGGAT ATAAGGCATCGCACGCTAAACATAACAGCCACACTTTCACTGCGGATTTG ACACTCGCAGGCAAACCGTGCGACACGTATGGCACCGATTTGAAGGACCT GAAGCTCTTAGTGGAGTACCAAACGGACGAACGCCTCCACGTCATGATCT ATGATGCCAATGAACAGGTGTACCAAGTTCCTGAGTCTGTTCTGCCTCGG GTTGGCAATGGCAATGGCACTGAGAAGGATTCTGCTCTGAAGTTCGATTA TGTGGAAGAGCCTTTCTCGTTCACCGTGTCTAGGAATGGAGATGTGCTTT TCGATACCTCTGCATCCAACCTGATCTTCCAATCACAGTATCTGAATCTT CGCACCTGGTTACCAAATGACCCTCATCTATATGGCCTCGGAGAGCATAC CGATTCGCTCCGATTGGAAACGAACAATTACACCCGTACCCTATGGAATC GTGATTCCTACGGCGTACCGAGCCACAGCAATCTGTACGGTGCTCACCCT GTGTACTACGACCATCGTGGGAGTGCTGGAACCCACGGTGTCTTCTTAGC AAATTCGAATGGTATGGACATCAAAATCAATAAGACACTGGATGGCAAGC AGTATCTTGAGTACAATATACTGGGAGGCGTACTTGATTTCTATTTCTTC ACCGGTTCGACTCCCAAGGAAGCTAGTACCCAGTACGCGAAAGTTGTTGG GCTTCCAGCCATGCAGAGCTACTGGACCTTTGGTTTTCATCAATGCAAGT ACGGTTATAGAGATGTTTATGAAGTGGCTGAAGTGGTGTATAATTATAGC CAAGCTGGAATCCCACTAGAGACCATGTGGACCGACATTGACTATATGGA GCTGAGAAGAGTGTTCACCCTCGACCCGGAAAGATTCCCACTTGGCAAAA TGCGGGAGCTTGTGGATTACCTTCATGACCATAATCAGCATTATATTGTC ATGGTTGATCCCGCCGTAAGCACGAGTGACAACCCGGGCTATCGGAGGGG AGTTGAGCAGGATATATTCCTCAAGACGCAGAACGGTAGTCTGTACAAAG GTGCTGTGTGGCCTGGTGTAACTGTCTACCCTGATTGGTTCCATCCAGCC ATTCAAGACTATTGGAACGGGGAGTTCAATAAGTTCTTCGATCCTGAAAC CGGTATCGACATTGACGGCCTGTGGATCGACATGAACGAGGCCGCAAATA TGTGCACCTTTCCCTGCACCGACCCGGAAAGATACTCTATTGAGAACGAT CTTCCACCCGCACCACCCGCTGTCCGGCCCAGTAACCCTCGTCCGTTGCC TGGATTCCCGGATGACTTCCAGCCAGGCTCTTCGAAGAGACTCTCGAAAA GAGCACATGGTGATAAGCTTGGGCTGCCTGGCCGCAACCTCCTCAGCCCC CCTTACTCAATTAAGAATGCGGCTGGAGCTCTTAGCCAGAACACTATTCA AACCAACATTGGCCACGCAGGAGGTTACGTTGAATACGACACCCATAATC TCTATGGAACCATGATGAGCTCGGCCTCTCGCATAGCAATGCAGCAACGC CGTCCCGACGTCAGACCCTTAATCATCACTCGCAGCACGTACGCAGGTGC CGGAGCACATGTTGGGCATTGGCTCGGTGACAATCTCAGCACTTGGAAGT TGTACCGCGCCTCGATCGCCCAGGTCCTGGCCTTCGCCTCGATGTTCCAG ATTCCCATGGTAGGAGCTGATGTCTGCGGCTTCGGTTCAAACACCACAGA AGAGCTCTGTGCGCGCTGGGCATCACTTGGAGCCTTCTACACCTTCTACC GTAACCATAACGAGATTGGCAACATCCCCCAGGAGTACTACTACTGGGAA TCGGTAACTGAATCCGCGACCAAGGCCATCAACATCCGGTACCAGCTCCT CGACTACGTTTACACTGCCTTCCACCGCCAGAGCAAAACCGGTGAGCCCT TCCTGCAGCCCTTATTCTACCTGTACCCAGAGGACAAAAATACCTTCGCA ATCGACCTGCAGTTTTTCTACGGCGACGCCATTCTTATCTCCCCCGTCAC CGAGAAAAACTCGACATCCGTCAATGCATATTTTCCCAAGGATATCTTCT ACGATTGGTACACCGGCGCCGTAATCCAGGGTCAGGGCGCGAATATCATT CTGTCCAATATCAACATCACCCATATCCCCATTCACATCCGTGGCGGCAA TATCGTCCCCATCCGCTCCTCTGGTGCGATGACAACCACCGAGCTCCGCA AAAAGGGCTTCCAGCTTATCATTGCTTCTGGCATCGATGGGACAGCTTCT GGAAGCCTGTATTTAGACGATGGAGACTCGCTCGAGCAGACTGACACTGC CAACATTGAGTTCGAGTACCGCAGTGGTGTTCTCTACATCAAGGGGCAAT TTATCCACGATGTCCCTGTTAAGATTGAGAGTGTTATTTTGCTTGGTCAG ACAACGACTGCCGGGGCTGGGCGGCACAACCAGAAGCAGGTCATTGAAAC CAACTTGGAGCTTACGGCGCCTACGAGGGTAAAACTAGCTCTAGAACAAA AACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCAT CATCATTGA
Amino Acid Sequence (clone): SMNSRDMLGFLLFLPLVGAAVIGPRANSQICPGYKASHAKHNSHTFTADL TLAGKPCDTYGTDLKDLKLLVEYQTDERLHVMIYDANEQVYQVPESVLPR VGNGNGTEKDSALKFDYVEEPFSFTVSRNGDVLFDTSASNLIFQSQYLNL RTWLPNDPHLYGLGEHTDSLRLETNNYTRTLWNRDSYGVPSHSNLYGAHP VYYDHRGSAGTHGVFLANSNGMDIKINKTLDGKQYLEYNILGGVLDFYFF TGSTPKEASTQYAKVVGLPAMQSYWTFGFHQCKYGYRDVYEVAEVVYNYS QAGIPLETMWTDIDYMELRRVFTLDPERFPLGKMRELVDYLHDHNQHYIV MVDPAVSTSDNPGYRRGVEQDIFLKTQNGSLYKGAVWPGVTVYPDWFHPA IQDYWNGEFNKFFDPETGIDIDGLWIDMNEAANMCTFPCTDPERYSIEND LPPAPPAVRPSNPRPLPGFPDDFQPGSSKRLSKRAHGDKLGLPGRNLLSP PYSIKNAAGALSQNTIQTNIGHAGGYVEYDTHNLYGTMMSSASRIAMQQR RPDVRPLIITRSTYAGAGAHVGHWLGDNLSTWKLYRASIAQVLAFASMFQ IPMVGADVCGFGSNTTEELCARWASLGAFYTFYRNHNEIGNIPQEYYYWE SVTESATKAINIRYQLLDYVYTAFHRQSKTGEPFLQPLFYLYPEDKNTFA IDLQFFYGDAILISPVTEKNSTSVNAYFPKDIFYDWYTGAVIQGQGANII LSNINITHIPIHIRGGNIVPIRSSGAMTTTELRKKGFQLIIASGIDGTAS GSLYLDDGDSLEQTDTANIEFEYRSGVLYIKGQFIHDVPVKIESVILLGQ TTTAGAGRHNQKQVIETNLELTAPTRVKLALEQKLISEEDLNSAVDHHHH HH
Primers Used (Restriction sites): GAAAGCACGTGATATGTTGGGATTTCTACTTTTCC (Pml I) TACAGTCTAGAGCTAGTTTTACCCTCGTAGGC (Xba I)
pH optimum: 5.5
Specific activity (Substrate): PNP-alpha-glucoside
T optimum: 52
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMMY
Description: active on PNP-alpha-glucoside
References: not yet available