Enzyme Function:xyloglucan specific endoglucanase
Accession number: AN0452.2
GenBank ID: DQ490466
FGSC number: 10061
Average molecular weight
(clone, calculated): 		29.639 kDa
AA length (clone): 280 aa
DNA length (clone): 843 bp
DNA source: xylan cDNA
DNA Sequence (clone): TCGATGAATTCACGTGGCCCAGCCGGCCGTCTCGGATCGGTACCTCGAGC CGCGGATATGAAGCTGCTTGCTCTTTCCCTGGCCTCGTTGGCCTCCGCTG CCACCATCACTCGCCGTGCCGACTTCTGCGGCCAATGGGACACTGCCACG GCGGGTAACTTCATCGTATACAACAATCTCTGGGGTCAGGACAATGCCGA TAGTGGCTCGCAATGCACTGGTGTGGACTCCGCCAATGGCAACAGTGTCT CATGGCATACGACCTGGAGCTGGTCTGGTGGCTCCTCCAGTGTTAAAAGC TACGCGAATGCTGCGTATCAGTTCACGGCGACCCAGTTGAGCAGTCTGAG CTCGATCCCGTCTACCTGGGAATGGCAGTACTCTACGACCGATGTCGTTG CCAACGTTGCCTACGATCTCTTCACGAGCTCCAGCATTGGCGGAGACTCT GAGTACGAGATCATGATCTGGCTGGCCGCTCTGGGCGGTGCGGGCCCTAT CTCCTCGACCGGCTCTTCCATCGCAACGGTAACCCTTGGCGGAGTCACCT GGAACCTGTATTCCGGCCCCAACGGAAGCATGCAGGTGTACAGCTTCGTC GCATCATCCACCACTGAGAGCTTCTCGGCCGATCTGATGGACTTTATCAA TTACCTCGTGGAGAACCAAGGCCTGTCGAACAGCCAGTACCTGACTCACG TGCAGGCCGGTACCGAGCCATTCACTGGCTCCGACGCCACCTTGACTGTT TCCTCTTACTCAGTCTCTGTCTCTCTAGAACAAAAACTCATCTCAGAAGA GGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGA
Amino Acid Sequence (clone): SMNSRGPAGRLGSVPRAADMKLLALSLASLASAATITRRADFCGQWDTAT AGNFIVYNNLWGQDNADSGSQCTGVDSANGNSVSWHTTWSWSGGSSSVKS YANAAYQFTATQLSSLSSIPSTWEWQYSTTDVVANVAYDLFTSSSIGGDS EYEIMIWLAALGGAGPISSTGSSIATVTLGGVTWNLYSGPNGSMQVYSFV ASSTTESFSADLMDFINYLVENQGLSNSQYLTHVQAGTEPFTGSDATLTV SSYSVSVSLEQKLISEEDLNSAVDHHHHHH
Primers Used (Restriction sites): GAAAACCGCGGATATGAAGCTGCTTGCTCTTTC (Sac II) TACAGTCTAGAGAGACAGAGACTGAGTAAGAGGAA (Xba I)
pH optimum: 5.5
Specific activity (Substrate): PNP-beta-glucoside
T optimum: 52
ph Range Link: Link
Temperature Link: Link
Optimal Expression Medium: BMM
Description: active on tamarind XG, cuts after the unsubstituted glucose within the XG chain
References: not yet available